Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections Papers Received: 11 December 1978 Revised: 02 March 1979 DOI:
Cite this article as: Junqueira, L.C.U., Bignolas, G. & Brentani, R.R. Histochem J (1979) 11: 447. doi:10.1007/BF01002772 Synopsis
Sirius Red, a strong anionic dye, stains collagen by reacting, via its sulphonic acid groups, with basic groups present in the collagen molecule. The elongated dye molecules are attached to the collagen fibre in such a way that their long axes are parallel. This parallel relationship between dye and collagen results in an enhanced birefringency.
Examination of tissue sections from 15 species of vertebrates suggests that staining with Sirius Red, when combined with enhancement of birefringency, may be considered specific for collagen. An improved and modified method of staining with Sirius Red is presented.
Bancroft, J. D.
Theory and Practice of Histological Techniques
. pp. 129. Edinburgh: Churchill-Livingstone.
Constantine, V. S.
Mowry, R. W.
(1968). The selective staining of human dermal collagen. II. The use of Picrosirius Red F3BA with polarization microscopy.
J. invest. Derm.
Deitch, A. D.
Terney, J. Y.
(1965). Effect of acetylation on acid dye binding and Sakaguchi reaction.
J. Histochem. Cytochem.
Histochimie normale et pathologique
. pp. 1517. Paris: Gautier Villars.
Junqueira, L. C. U., Cossermelli, W. S.
Brentani, R. R.
(1978). Differential staining of collagens type I, II and III by Sirius Red and polarization microscopy.
Arch. histol. jap.
Junqueira, L. C. U., Bignolas, G. & Brentani, R. R. (1979). A simple and sensitive method for the quantitative estimation of collagen. Anayt. Biochem. (in press).
Mueller-Eberhard, H. J.
Ann. Rev. Biochem.
Puchtler, H., Waldrop, F. S.
Valentine, L. S.
(1973). Polarization microscopic studies of connective tissue stained with Picrosirius Red F3BA.
(1963). Masking action of basic proteins on sialic acid carboxyls in epithelial mucins.
Spicer, S. S.
(1962). Basic protein visualized histochemically in mucinous secretions.
Exp. Cell Res.
Stoward, P. J.
(1975). A histochemical study of the apparent deamination of proteins by sodium hypochlorite.
Sweat, F., Puchtler, H.
Rosenthal, S. I.
(1964). Sirius Red F3BA as stain for connective tissue.
Freedberg, I. M.
(1972). Epidermal structural proteins. Isolation and purification of keratohyalin granules of newborn rat.
Biochim. Biophys. Acta
The Physical Chemistry of Dyeing
. London: Oliver and Boyd.
Weatherford, T. W.
(1972). Staining of collagenous and non-collagen structures with Picrosirius Red F3BA.
Ala. J. Med. Sci.
Google Scholar Copyright information
© Chapman and Hall Ltd. 1979