The Histochemical Journal

, Volume 11, Issue 4, pp 447–455

Picrosirius staining plus polarization microscopy, a specific method for collagen detection in tissue sections

Authors

  • L. C. U. Junqueira
    • Laboratórios de Biologia Celular, Pesquisa em Reumatologia e Oncologia Experimental, Faculdade de MedicinaUniversidade de São Paulo
  • G. Bignolas
    • Laboratórios de Biologia Celular, Pesquisa em Reumatologia e Oncologia Experimental, Faculdade de MedicinaUniversidade de São Paulo
  • R. R. Brentani
    • Laboratórios de Biologia Celular, Pesquisa em Reumatologia e Oncologia Experimental, Faculdade de MedicinaUniversidade de São Paulo
Papers

DOI: 10.1007/BF01002772

Cite this article as:
Junqueira, L.C.U., Bignolas, G. & Brentani, R.R. Histochem J (1979) 11: 447. doi:10.1007/BF01002772

Synopsis

Sirius Red, a strong anionic dye, stains collagen by reacting, via its sulphonic acid groups, with basic groups present in the collagen molecule. The elongated dye molecules are attached to the collagen fibre in such a way that their long axes are parallel. This parallel relationship between dye and collagen results in an enhanced birefringency.

Examination of tissue sections from 15 species of vertebrates suggests that staining with Sirius Red, when combined with enhancement of birefringency, may be considered specific for collagen. An improved and modified method of staining with Sirius Red is presented.

Download to read the full article text

Copyright information

© Chapman and Hall Ltd. 1979