Molecular Biology Reports

, Volume 22, Issue 1, pp 47–52

Interaction of Co, Mn, Mg and Al with d(GCGTACGC): a spectroscopic study

Authors

  • M. T. Rajan
    • Department of Biochemistry and NutritionCentral Food Technological Research Institute
  • D. Champion
    • Department of PhysicsIndian Institute of Science
    • The Jawaharlal Nehru Center for Advanced Scientific Research
  • C. S. Kumar
    • Department of PhysicsIndian Institute of Science
    • The Jawaharlal Nehru Center for Advanced Scientific Research
  • D. Vishnuvardhan
    • Department of Biochemistry and NutritionCentral Food Technological Research Institute
  • K. S. Jagannatha Rao
    • Department of Biochemistry and NutritionCentral Food Technological Research Institute
  • M. A. Viswamitra
    • Department of PhysicsIndian Institute of Science
    • The Jawaharlal Nehru Center for Advanced Scientific Research
Research Articles

DOI: 10.1007/BF00996304

Cite this article as:
Rajan, M.T., Champion, D., Kumar, C.S. et al. Mol Biol Rep (1995) 22: 47. doi:10.1007/BF00996304

Abstract

Spectroscopic study on the interactions of trace elements Co, Mn, Mg and Al with d(GCGTACGC) indicated the following: Al and Mg did not alter Tm values. Mn enhanced Tm at lower concentration and decreased it at higher concentrations. Interestingly Co at higher concentration elevated the Tm. These studies also showed lower concentrations of Mn displaced EtBr, whereas Al could displace it at higher ionic strength. Mg and Co displaced EtBr fluorescence at moderate concentrations. The binding constant values and CD spectra clearly indicated strong binding of these elements to DNA.

Key words

DNAinteractionspectroscopy and trace elements

Copyright information

© Kluwer Academic Publishers 1996