Evidence for diglycerides as attractants in an ant-seed interaction
Received: 20 April 1978 Revised: 11 August 1978 DOI:
10.1007/BF00987919 Cite this article as: Marshall, D.L., Beattie, A.J. & Bollenbacher, W.E. J Chem Ecol (1979) 5: 335. doi:10.1007/BF00987919 Abstract
The chemical basis of an ant-seed interaction was investigated for the ant
Aphaenogaster rudis and the ant-dispersed violet Viola odorata. A laboratory behavioral bioassay was developed to chemically identify the attractant responsible for the interaction. The ant attractant, localized in the elaiosome, was classified as a lipid by both field and laboratory bioassays. Assays of partially purified lipid extracts revealed that the principal attractant may be a diglyceride. Gas-liquid chromatography analysis of the hydrolyzed diglyceride fraction revealed oleic acid as the major fatty acid present, suggesting that either 1,2- or 1,3-diolein may be the attractant. Structure-activity correlations for lipid standards demonstrated a clear preference for the diglyceride 1,2-diolein. The data also suggest that ricinoleic acid is not the lipid eliciting the ant response to Viola odorata, as had been previously suggested. Key words Hymenoptera Formicidae Aphaenogaster rudis ant behavior diglyceride elaiosome myrmecochory Viola odorata References
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© Plenum Publishing Corporation 1979