Plant Systematics and Evolution

, Volume 190, Issue 3, pp 187–193

Intergenic spacers of rRNA genes in three species of theCynareae (Asteraceae)

Authors

  • Gianfranco Tucci
    • D.A.B.A.C.Universitá degli Studi della Tuscia
  • Marco Cosimo Simeone
    • D.A.B.A.C.Universitá degli Studi della Tuscia
  • Carlo Gregori
    • D.A.B.A.C.Universitá degli Studi della Tuscia
  • Fabio Maggini
    • D.A.B.A.C.Universitá degli Studi della Tuscia
Article

DOI: 10.1007/BF00986192

Cite this article as:
Tucci, G., Simeone, M.C., Gregori, C. et al. Pl Syst Evol (1994) 190: 187. doi:10.1007/BF00986192

Abstract

Intergenic spacers of the rRNA genes of three species of theCynareae tribe:Cynara cardunculus subsp.scolymus (artichoke),Onopordum acanthium, andO. illyricum were cloned in the plasmid pGEM-7zf(+). Detailed restriction mapping and partial sequencing of the IGSs were carried out. The structural analysis showed a clear diversity betweenCynara andOnopordum, while a high degree of homology was found between the twoOnopordum spp. In all three species a fragment of about 450 bp from the 5′ end of 18S to the Acc I site with a high sequence homology was present. Nucleotide sequences upstream from the above mentioned Acc I site show a gradual decrease of homology betweenCynara andOnopordum.

Key words

AsteraceaeCynareaerDNAintergenic spacercloningsequencing

Copyright information

© Springer-Verlag 1994