Article

Plant Systematics and Evolution

, Volume 190, Issue 3, pp 187-193

First online:

Intergenic spacers of rRNA genes in three species of theCynareae (Asteraceae)

  • Gianfranco TucciAffiliated withD.A.B.A.C., Universitá degli Studi della Tuscia
  • , Marco Cosimo SimeoneAffiliated withD.A.B.A.C., Universitá degli Studi della Tuscia
  • , Carlo GregoriAffiliated withD.A.B.A.C., Universitá degli Studi della Tuscia
  • , Fabio MagginiAffiliated withD.A.B.A.C., Universitá degli Studi della Tuscia

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Abstract

Intergenic spacers of the rRNA genes of three species of theCynareae tribe:Cynara cardunculus subsp.scolymus (artichoke),Onopordum acanthium, andO. illyricum were cloned in the plasmid pGEM-7zf(+). Detailed restriction mapping and partial sequencing of the IGSs were carried out. The structural analysis showed a clear diversity betweenCynara andOnopordum, while a high degree of homology was found between the twoOnopordum spp. In all three species a fragment of about 450 bp from the 5′ end of 18S to the Acc I site with a high sequence homology was present. Nucleotide sequences upstream from the above mentioned Acc I site show a gradual decrease of homology betweenCynara andOnopordum.

Key words

Asteraceae Cynareae rDNA intergenic spacer cloning sequencing