Plant Systematics and Evolution

, Volume 214, Issue 1, pp 49–64

The chloroplast genome arrangement ofLobelia thuliniana (Lobeliaceae): Expansion of the inverted repeat in an ancestor of theCampanulales

  • Eric B. Knox
  • Jeffrey D. Palmer
Article

DOI: 10.1007/BF00985731

Cite this article as:
Knox, E.B. & Palmer, J.D. Pl Syst Evol (1999) 214: 49. doi:10.1007/BF00985731

Abstract

A clone-bank ofSac I restriction fragments was constructed from the chloroplast DNA (cpDNA) ofLobelia thulinianaE. B. Knox (Lobeliaceae). These cloned fragments and a set of 106 clones spanning the tobacco chloroplast genome were used as probes to determine the cpDNA restriction fragment arrangement forSac I and six other restriction enzymes (BamH I,EcoR V,Hind III,Nci I,Pst I, andXho I) and the chloroplast genome arrangement ofL. thuliniana relative to tobacco, which has been fully sequenced and is collinear with the hypothesized ancestral genome arrangement of angiosperms. The results confirm and refine our previous understanding of the chloroplast genome arrangement in the large single-copy region (LSC) and reveal (1) a roughly 11 kilobase (kb) expansion of the inverted repeat (IR) into the small single-copy region (SSC) and (2) apparent sequence divergence of the DNA segment inL. thuliniana that corresponds to ORF1901 in tobacco. The expansion of the IR into the SSC is present in all other examined members ofLobeliaceae, Cyphiaceae, andCampanulaceae, which indicates that the IR expansion was an early event in the cpDNA evolution of theCampanulales. The IR expansion into the SSC was not present inSphenoclea, which additionally supports exclusion of this genus from theCampanulaceae.

Key words

Campanulales Campanulaceae Cyphiaceae Lobeliaceae Sphenocleaceae Lobelia thuliniana Chloroplast DNA (cpDNA) inverted repeat (IR) genome rearrangements 

Copyright information

© Springer-Verlag 1999

Authors and Affiliations

  • Eric B. Knox
    • 1
  • Jeffrey D. Palmer
    • 2
  1. 1.Department of Biological SciencesRutgers UniversityNewarkUSA
  2. 2.Department of BiologyJordan Hall Indiana UniversityBloomingtonUSA