Plant Systematics and Evolution

, Volume 223, Issue 1, pp 71–79

Cytological characterization of heterochromatin and rDNA inPinus radiata andP. taeda

  • M. D. Jacobs
  • R. C. Gardner
  • B. G. Murray
Article

DOI: 10.1007/BF00985327

Cite this article as:
Jacobs, M.D., Gardner, R.C. & Murray, B.G. Pl Syst Evol (2000) 223: 71. doi:10.1007/BF00985327

Abstract

Fluorochrome C-banding ofPinus radiata andP. taeda metaphase chromosomes showed many pericentromeric DAPI bands and interstitial CMA bands inP. radiata, and centromeric and interstitial CMA bands inP. taeda. Giemsa C-band patterns differed between the species with centromeric bands inP. radiata but no consistent bands inP. taeda. A karyotype ofP. radiata was developed based on banding patterns that distinguished all but two of the 12 pairs of chromosomes. In situ hybridization (ISH) using probes for high-copy ribosomal DNA (rDNA) showed 10 pairs of 18S–25S sites and two pairs of 5S sites in both species. Most of the sites were interstitial or centromeric.

Key words

Pinus radiata P. taeda Heterochromatin ribosomal RNA chromosome banding in situ hybridization karyotype 

Copyright information

© Springer-Verlag 2000

Authors and Affiliations

  • M. D. Jacobs
    • 1
  • R. C. Gardner
    • 1
  • B. G. Murray
    • 1
  1. 1.School of Biological SciencesThe University of AucklandAucklandNew Zealand