Plant Systematics and Evolution

, Volume 203, Issue 1, pp 27–40

Chromosome analysis and DNA homology in threePicea species,P. mariana, P. rubens, andP. glauca (Pinaceae)

  • K. K. Nkongolo
Article

DOI: 10.1007/BF00985235

Cite this article as:
Nkongolo, K.K. Pl Syst Evol (1996) 203: 27. doi:10.1007/BF00985235

Abstract

A detailed karyotype analysis was made on the somatic complement ofPicea rubens andP. glauca. B-chromosomes were observed in someP. glauca populations. The karyotypes are generally asymmetrical with most of the chromosomes having median to median-submedian centromeres.Picea glauca chromosomes 2, 3, 7, and 8 have secondary constriction on their short arm and chromosome 10 has a secondary constriction on the long arm. Chromosome 3 was the most easily identifiable, as it has two secondary constrictions located on the short arm. InP. rubens, all the chromosomes but chromosomes 8 and 9 have one to four distinctive secondary constrictions. In general, the diagrammatic comparisons show a high degree of similarity amongP. mariana, P. rubens, andP. glauca. GenomicP. mariana probe strongly hybridized to dots of genomic DNA fromP. rubens andP. glauca indicating that there is a high sequence homology among these three species. The synchronizing agent, hydroxyurea was used at different concentrations to enhance the mitotic index of cell suspensions derived from embryogenic cultures. Hydroxyurea at 1.25 mM increased significantly the mitotic index. An increase of hydroxyurea from 1.25 mM to 5 mM and 10 mM resulted in a steady decrease of mitotic index.

Key words

PinaceaePiceaSprucemitotic indexcytotaxonomykaryotypeDNA homology

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • K. K. Nkongolo
    • 1
  1. 1.Department of Biological SciencesLaurentian UniversitySudburyCanada