Application of current chemical concepts to metal-hematein and-brazilein stains
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- Puchtler, H., Meloan, S.N. & Waldrop, F.S. Histochemistry (1986) 85: 353. doi:10.1007/BF00982665
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Without differentiation or acid in dye chelate solutions, staining patterns are a function of the metal. Reactions of acidified solutions are determined by the affinities of the dye ligands. Brazilein is much more acid-sensitive than hematein. This difference can be ascribed to the lack of a second free phenolic −OH group in brazilein, i.e. one hydrogen bond is insufficient to anchor the dye to tissues. Since hematein and brazilein are identical in all other respects, their differences in affinity cannot be explained by van der Waals, electrostatic, hydrophobic or other forces.