Journal of Chemical Ecology

, Volume 18, Issue 7, pp 931–944

6-Methoxy-2-benzoxazolinone: A semiochemical for host location by western corn rootworm larvae

  • Louis B. Bjostad
  • Bruce E. Hibbard
Article

DOI: 10.1007/BF00980054

Cite this article as:
Bjostad, L.B. & Hibbard, B.E. J Chem Ecol (1992) 18: 931. doi:10.1007/BF00980054

Abstract

A bioassay-driven sequential fractionation scheme was used to isolate all portions of a crude dichloromethane corn seedling extract behaviorally active to larvae of the western corn rootworm,Diabrotica virgifera virgifera LeConte. 6-Methoxy-2-benzoxazolinone (MBOA) was identified as one of the most important components of an attractive crude corn extract. MBOA was found on or in the intact root tissues by injecting an extract of undamaged roots onto an HPLC immediately after extraction. MBOA was demonstrated to be volatile and functions as a semiochemical in conjunction with carbon dioxide in host location by western corn rootworm larvae, which are oligophagous on the roots of maize and several other species of grasses. Because MBOA occurs almost exclusively in maize and other grasses, it offers a simple way for the larvae to distinguish possible hosts from non-hosts. MBOA has previously been reported as a chemical defense against other insect species. This is the first report in grasses of a secondary compound that is toxic or a deterrent to nonadapted insect herbivores but that is used as a semiochemical in host location by a specialist insect species.

Key Words

Diabrotica virgifera virgifera6-methoxy-2-benzoxazolinonehydroxamic acidssemiochemicalattractantswestern corn rootwormhost locationColeopteraChrysomelidaeZea mayskairomone

Copyright information

© Plenum Publishing Corporation 1992

Authors and Affiliations

  • Louis B. Bjostad
    • 1
  • Bruce E. Hibbard
    • 1
  1. 1.Department of EntomologyColorado State UniversityFort Collins