Neurochemical Research

, Volume 13, Issue 3, pp 203–207

Anti-galactocerebroside antibodiesin human cerebrospinal fluids determined by enzyme-linked immunosorbent assay (ELISA)

  • Takao Ichioka
  • Ken-ichiro Uobe
  • Michael Stoskopf
  • Yasuo Kishimoto
  • Gihan Tennekoon
  • Wallace W. Tourtellotte
Original Articles

DOI: 10.1007/BF00971533

Cite this article as:
Ichioka, T., Uobe, K., Stoskopf, M. et al. Neurochem Res (1988) 13: 203. doi:10.1007/BF00971533

Abstract

The standard ELISA technique was improved for the detection of antigalactocerebroside antibody in biological fluid. Mouse monoclonal antigalactocerebroside antibody was used to demonstrate specificity and sensitivity of the technique. After optimization of the assay, the usefulness of this measurement for the evaluation of patients with multiple sclerosis was assessed. The presence of antigalactocerebroside antibodies in the cerebrospinal fluid of 20 patients with multiple sclerosis, 10 with other neurological diseases and 10 normal individuals was determined. All the CSF samples from normal individuals were negative. In patients with multiple sclerosis 14 of the 20 samples had elevated levels of antigalactocerebroside antibody, whereas with other neurological diseases 5 out of 10 were positive. Antigalactocerebroside levels were lower in samples from patients during an acute relapse than in those from more chronic cases. These results indicate that the presence of anti-galactocerebroside antibody in cerebrospinal fluid is not specific to MS but may reflect previous damage to myelin.

Key Words

Antibodymyelincerebrosidemultiple sclerosisELISAcerebrospinal fluid

Abbreviations and trivial names used

ELISA

Enzyme-Linked Immunosorbent Assay

CSF

cerebrospinal fluid; galacto- or glucocerebroside, ceramide-1-0-beta-galactoside or-glucoside

Copyright information

© Plenum Publishing Corporation 1988

Authors and Affiliations

  • Takao Ichioka
    • 1
    • 2
  • Ken-ichiro Uobe
    • 1
    • 2
  • Michael Stoskopf
    • 1
    • 3
  • Yasuo Kishimoto
    • 1
    • 2
  • Gihan Tennekoon
    • 2
  • Wallace W. Tourtellotte
    • 4
  1. 1.The Kennedy Institute, School of MedicineJohns Hopkins UniversityBaltimore
  2. 2.Department of Neurology School of MedicineJohns Hopkins UniversityBaltimore
  3. 3.Department of Contparative Medicine, School of MedicineJohns Hopkins UniversityBaltimore
  4. 4.Neurology Service Veterans Administration, Wadsworth Medical Center and Department of NeurologyUniversity of CaliforniaLos Angeles
  5. 5.Department of PediatricsTokushima University School of MedicineTokushimaJapan
  6. 6.Department of Oral PathologyOsaka Dental UniversityOsakaJapan