Neurochemical Research

, Volume 10, Issue 8, pp 1107–1118

Immunohistochemical localization of 2′,3′-cyclic nucleotide 3′-phosphodiesterase in adult bovine cerebrum and cerebellum

  • Yoshiko Nishizawa
  • Tadashi Kurihara
  • Toru Masuda
  • Yasuo Takahashi
Original Articles

DOI: 10.1007/BF00965885

Cite this article as:
Nishizawa, Y., Kurihara, T., Masuda, T. et al. Neurochem Res (1985) 10: 1107. doi:10.1007/BF00965885

Abstract

We have previously shown that 2′,3′-cyclic nucleotide 3′-phosphodiesterase (CNP; EC 3.1.4.37) in rat central nervous tissues can be immunohistochemically stained with anti-bovine CNP serum. However, the anti-bovine CNP serum prepared in our laboratory has only weak cross-reactivity with rat CNP. Sections of bovine nervous tissues were found to be stained effectively with the serum, and the localization of CNP has been revealed in greater detail. We describe here the immunohistochemical localization of CNP in adult bovine cerebrum and cerebellum. CNP stained was localized in myelin sheaths, oligodendrocytes, and the processes of oligodendrocytes; astrocytes and neurons were negative. All myelinated nerve fibers appeared to be stained with the anti-CNP serum. Perineuronal and perivascular oligodendrocytes, and oligodendrocytes extending their processes to isolated myelin fibers were stained. Interfascicular oligodendrocytes, however, did not react or reacted faintly to the anti-CNP serum; only their processes were reactive. Comparison with the stain for S-100 protein was helpful to distinguish oligodendrocytes from astrocytes particularly when both glial cells were situated together at the perineuronal and perivascular positions.

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Yoshiko Nishizawa
    • 2
  • Tadashi Kurihara
    • 1
  • Toru Masuda
    • 3
  • Yasuo Takahashi
    • 3
  1. 1.Department of Neuropharmacology Brain Research InstituteNiigata UniversityNiigataJapan
  2. 2.National Saigata Sanatorium and Rehabilitation CenterNiigata-kenJapan
  3. 3.Department of Anesthesiology, Faculty of MedicineToyama Medical and Pharmaceutical UniversityToyamaJapan