Molecular and Cellular Biochemistry

, Volume 144, Issue 2, pp 125–129

Protective effect of spermine on DNA exposed to oxidative stress

  • Claudio Muscari
  • Carlo Guarnieri
  • Claudio Stefanelli
  • Antonella Giaccari
  • Claudio Marcello Caldarera
Article

DOI: 10.1007/BF00944391

Cite this article as:
Muscari, C., Guarnieri, C., Stefanelli, C. et al. Mol Cell Biochem (1995) 144: 125. doi:10.1007/BF00944391

Abstract

Pathological conditions that cause oxidative stress can affect DNA integrity. The aim of this research was to study the protective effect of spermine against DNA damage induced by an oxygen-radical generating system. Deoxyguanosine and DNA were separately dissolved in phosphate buffer and incubated for 1 h at 40°C in the presence of 50 mMH2O2/10 mM ascorbic acid. Single nucleosides and their products of oxidation were then obtained by enzymatic digestion of DNA. The compounds were separated by micellar electrokinetic capillary chromatography (MECC) with SDS-modified mobile phase and detected at 254 nm. Two major products of DNA oxidation have been identified as derivatives of deoxyguanosine with electrophoretic properties different from 8-hydroxy-2′-deoxyguanosine. When the oxidation of DNA was carried out in the presence of 0.1 mM spermine, the formation of the two by-products of deoxyguanosine was markedly reduced. On the contrary, spermine did not prevent the oxidation of deoxyguanosine alone, suggesting that the polyamine should be bound to the DNA strands to exert its antioxidative effect.

Key words

DNA oxidation oxygen free radical 8-hydroxy-2′-deoxyguanosine polyamine spermine capillary electrophoresis 

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Claudio Muscari
    • 1
  • Carlo Guarnieri
    • 1
  • Claudio Stefanelli
    • 1
  • Antonella Giaccari
    • 1
  • Claudio Marcello Caldarera
    • 1
  1. 1.Center of Research on Heart Metabolism, Department of BiochemistryUniversity of BolognaItaly
  2. 2.Dipartimento di BiochimicaUniversita' di BolognaBolognaItaly

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