Anatomy and Embryology

, Volume 184, Issue 2, pp 103–124

Ontogeny of the calcium binding protein parvalbumin in the rat nervous system

  • Sven Solbach
  • Marco R. Celio
Article

DOI: 10.1007/BF00942742

Cite this article as:
Solbach, S. & Celio, M.R. Anat Embryol (1991) 184: 103. doi:10.1007/BF00942742

Summary

In the adult rat brain, the calcium-binding protein parvalbumin is preferentially associated with spontaneously fast-firing, metabolically active neurons and coexists with gamma-amino-butyric acid (GABA) in cortical inhibitory interneurons. Whether this is so in developing neurons has not been explored. To this end, we have used parvalbumin immunohistochemistry to study expression of this protein in the rat nervous system during pre- and postnatal life. Our results indicate that parvalbumin first appears at embryonic day 13 in sensory system of the spinal cord, in the vestibular (VIII), the trigeminal (V) and the visuomotor (III, IV VI) systems, and develops rapidly during the following days. In these locations the expression of parvalbumin coincides with the beginning of physiological activity in nerve cells. In the gamma-aminobutyric acid (GABA)-containing interneurons of the cerebral cortex and the hippocampus, as well as in the Purkinje cells of the cerebellum, parvalbumin only appears postnatally. It lags behind the development of GABA-immunoreactivity by 1 to 2 weeks. The beginning of its expression, in the cerebellum at least, coincides with the arrival of excitatory synaptic input and the onset of spontaneous activity. Thus, during the development of the nervous system, the expression of parvalbumin is subordinate to the establishment of physiological activity.

Key words

Ca2+Visuomotor systemVestibular systemSomatosensory systemGABA-interneuronsNeuronal marker

Abbreviations

3

oculomotor nucleus

4

trochlear nucleus

4n

trochlear nerve

6

abducens nucleus

12

hypoglossal nucleus

3n

oculomotor nerve

4V

4th ventricle

5g

trigeminal ganglion

5n

trigeminal nerve

5mx

trigeminal nerve, maxillary branch

8c1

cochlear ganglion

8g

vestibular ganglion

8n

vestibular nerve

10n

vagal nerve

Amb

ambiguus nucleus

CaBP

calcium-binding protein

Ce

cerebellum

ChP

choroid plexus

cl

cochlea

CPu

caudate putamen

Cu

cuneate nucleus

Cx

cerebral cortex

df

dorsal funiculus spinal cord

dr

dorsal root spinal nerve

E15

embryonic day 15 of gestation

ECN

external cuneate nucleus

Fr

formatio reticularis

GABA

gamma-amino-butyric acid

GAD

glutamate decarboxylase

gl

granular layer cerebellum

Gr

gracile nucleus

Hip

hippocampus

H

heart

inc

inferior colliculus

IOK

inferior olive, kap cooy medial nucleus

Li

liver

LMol

lacunosum moleculare layer hippocampus

Lu

lung

LV

lateral ventricle

LVe(v)

lateral vestibular nucleus (ventral)

LVe(d)

lateral vestibular nucleus (dorsal)

me5

mesencephalic trigeminal tract

Me5

mesencephalic trigeminal nucleus

Mes

mesencephalon

ml

molecular layer cerebellum

MVe

medial vestibular nucleus

Or

oriens layer hippocampus

P 2

postnatal day 2

Pu

Purkinje-cell layer, cerebellum

Py

pyramidal cell layer, hippocampus

R

red nucleus

Rhom

rhombencephalon

Rt

reticular thalamic nucleus

sk

skin

sn

substantia nigra

spgl

spinal ganglion

sp5

spinal trigeminal tract

SpVe

spinal vestibular nucleus

Sp5I

spinal trigeminal nucleus, interpolar

suc

superior colliculus

SuVe

superior vestibular nucleus

TBS

tris-buffered saline

tch

tactile hair sinus

ve

vestibular epithelium

vb

vertebral body

vh

ventral horn spinal cord

VL

ventrolateral thalamic nucleus

VP

ventral pallidum

vr

ventral root spinal nerve

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Sven Solbach
    • 1
  • Marco R. Celio
    • 1
  1. 1.Institute of AnatomyChristian-Albrechts-University KielKielGermany
  2. 2.Institute of HistologyUniversity of FribourgFribourgSwitzerland