, Volume 66, Issue 1, pp 17-24

Stimulating effect of citrate and cis-aconitate on the transformation ofTrypanosoma brucei bloodstream forms to procyclic forms in vitro

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Abstract

Transformation ofTrypanosoma brucei bloodstream forms to procyclic forms can be stimulated in vitro by adding 2–10 mM citrate and/or cis-aconitate to the culture medium for at least 3 h. Optimal transformation was obtained in the presence of 3 mM cis-aconitate for 48 h. Citrate and cis-aconitate may activate isocitrate dehydrogenase and/or other Krebs' cycle enzymes and thus act as a metabolic trigger for the morphological changes involved in transformation.