Zeitschrift für Parasitenkunde

, Volume 66, Issue 1, pp 17–24

Stimulating effect of citrate and cis-aconitate on the transformation ofTrypanosoma brucei bloodstream forms to procyclic forms in vitro


  • R. Brun
    • Swiss Tropical Institute
  • M. Schönenberger
    • Swiss Tropical Institute
Original Investigations

DOI: 10.1007/BF00941941

Cite this article as:
Brun, R. & Schönenberger, M. Z. Parasitenkd. (1981) 66: 17. doi:10.1007/BF00941941


Transformation ofTrypanosoma brucei bloodstream forms to procyclic forms can be stimulated in vitro by adding 2–10 mM citrate and/or cis-aconitate to the culture medium for at least 3 h. Optimal transformation was obtained in the presence of 3 mM cis-aconitate for 48 h. Citrate and cis-aconitate may activate isocitrate dehydrogenase and/or other Krebs' cycle enzymes and thus act as a metabolic trigger for the morphological changes involved in transformation.

Copyright information

© Springer-Verlag 1981