Original Papers

Urological Research

, Volume 25, Issue 1, pp 59-65

First online:

Morphological analysis of renal cell culture models of calcium phosphate stone formation

  • Y. NaitoAffiliated withFirst Department of Pathology, Hamamatsu University School of Medicine
  • , Y. OhtawaraAffiliated withDepartment of Urology, Hamamatsu University School of Medicine
  • , S. KageyamaAffiliated withDepartment of Urology, Hamamatsu University School of Medicine
  • , M. NakanoAffiliated withDepartment of Urology, Hamamatsu University School of Medicine
  • , A. IchiyamaAffiliated withFirst Department of Biochemistry, Hamamatsu University School of Medicine
  • , M. FujitaAffiliated withSecond Department of Biochemistry, Hamamatsu University School of Medicine
  • , K. SuzukiAffiliated withDepartment of Urology, Hamamatsu University School of Medicine
  • , K. KawabeAffiliated withDepartment Urology, Faculty of Medicine, University of Tokyo
  • , I. KinoAffiliated withFirst Department of Pathology, Hamamatsu University School of Medicine

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Abstract

Cell culture models of calcium phosphate renal stone formation were established using the MDCK cell line. Renal microliths were detected within pseudocysts in three-dimensional soft agar cultures, and were also observed in the basal region of cells lining the cell sheet, and immediately beneath domes or blisters in monolayers and collagen gel cultures. Light and scanning electron microscopy indicated that these microliths had a similar lamellated and spherical appearance to those in humans. These microliths were first detected microscopically after 21 days of culture, and were found to be composed of calcium phosphate by X-ray and microinfrared spectroscopic analyses. These culture models may provide a powerful new tool to study the pathogenesis of renal stone diseases and/or calcium phosphate stone formation in humans and animals.

Key words

Renal stone Cell culture models MDCK cell line Calcium phosphate