Applied Microbiology and Biotechnology

, Volume 29, Issue 2, pp 107-112

First online:

Estimation of oxygen penetration depth in immobilized cells

  • Ho Nam ChangAffiliated withDepartment of Chemical Engineering, University of Waterloo
  • , Murray Moo-YoungAffiliated withDepartment of Chemical Engineering, University of Waterloo

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A simple method is proposed for calculating oxygen pentration depth in immobilized cells by assuming zero order kinetics in the presence of several external oxygen transport resistances. Calculations indicate that typical penetration depths of oxygen for immobilized microbial cells are in the range of 50–200 μ and those for immobilized or encapsulated animal and plant tissue culture are about 500–1000 μ. Based on calculations, oxygen transport in microencapsulation and microcarriers for tissue cultures are not transport-limited, but a slight limitation is expected for those in a hollow fiber reactor.