Applied Microbiology and Biotechnology

, Volume 24, Issue 2, pp 106–112

Effect of different limitations in chemostat cultures on growth and production of exocellular protease byBacillus licheniformis

Authors

  • Jurjen Frankena
    • Department of Microbiology, Biological LaboratoryVrije Universiteit
  • Gregory M. Koningstein
    • Department of Microbiology, Biological LaboratoryVrije Universiteit
  • Henk W. van Verseveld
    • Department of Microbiology, Biological LaboratoryVrije Universiteit
  • Adriaan H. Stouthamer
    • Department of Microbiology, Biological LaboratoryVrije Universiteit
Biotechnology

DOI: 10.1007/BF00938779

Cite this article as:
Frankena, J., Koningstein, G.M., van Verseveld, H.W. et al. Appl Microbiol Biotechnol (1986) 24: 106. doi:10.1007/BF00938779

Summary

Maximal molar growth yields (Ysubmax) and protease production ofBacillus licheniformis S 1684 during NH4+-, O2-, and NH4++O2-limitation with either glucose or citrate as carbon and energy source and during glucose-, and citratelimitation in chemostat cultures were determined. Protease production was repressed by excess ammonia when glucose served as C/E-source. Glucose and citrate repressed protease production during NH4+-limitation. A low oxygen tension enbanced protease production at low μ-values. It was concluded that, besides ammonia repression, catabolite flux and oxygen tension influence protease production, indicating that the energy status of the cell is important for the level of protease production.Ysubmax-values were high during glucose-limitation and indicate a high efficiency of growth caused by a highYATPmax. During NH4+-, O2-, and NH4++O2-limitation with glucose as C/E-values were lower than during glucose limitation. The lowerYsubmax-values were due to a lower efficiency of energy conservation.Ysubmax-values during limitations with citrate as C/E-source were lower than during limitations with glucose as C/E-source.

Nomenclature

μ

specific growth rate (h-1)

Ysub

growth yield per mol substrate (g biomass/mol)

Ymax

maximal molar growth yield corrected for maintenance requirements (g biomass/mol)

Ymax (corr)

Ymax corrected for product formation (g biomass/mol)

msub

maintenance requirements (mol/g biomass·h)

msub (corr)

maintenance requirements corrected for product formation (mol/g biomass·h)

qportmax

maximal specific rate of protease production (E440/mg DW·h)

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Copyright information

© Springer-Verlag 1986