Molecular and Cellular Biochemistry

, Volume 125, Issue 2, pp 137–143

Cloning and analysis of the human myocardial Na+/H+ exchanger

Authors

  • L. Fliegel
    • Departments of Biochemistry and Pediatrics, Faculty of MedicineUniversity of Alberta
  • J. R. B. Dyck
    • Departments of Biochemistry and Pediatrics, Faculty of MedicineUniversity of Alberta
  • H. Wang
    • Departments of Biochemistry and Pediatrics, Faculty of MedicineUniversity of Alberta
  • C. Fong
    • Departments of Biochemistry and Pediatrics, Faculty of MedicineUniversity of Alberta
  • R. S. Haworth
    • Departments of Biochemistry and Pediatrics, Faculty of MedicineUniversity of Alberta
Article

DOI: 10.1007/BF00936442

Cite this article as:
Fliegel, L., Dyck, J.R.B., Wang, H. et al. Mol Cell Biochem (1993) 125: 137. doi:10.1007/BF00936442

Abstract

The Na+/H+ exchanger is an integral membrane protein that is universally distribute in mammalian tissues and is responsible for intracellular pH regulation. Several isoforms of the Na+/H+ exchanger exist (NHE-1-NHE-4). The first that was cloned is the amiloride sensitive isoform (NHE-1). Using a fragment of the rabbit cardiac Na+/H+ exchanger cDNA clone we isolated and sequenced Na+/H+ exchanger cDNA from a human heart coding for the complete human Na+/H+ exchanger (NHE-1 isoform). Two overlapping cDNA clones were obtained, giving a combined sequence that contained both 3′ and 5′ untranslated regions. The 5′ and 3′ untranslated regions proved to be highly homologous to human sequences described earlier but contained some variations that could affect the mRNA stability and/or the efficiency of translation of the Na+/H+ exchanger. Northern blot analysis and reverse transcriptase polymerase chain reaction confirmed the presence of the 5 kb NHE-1 message in primary cultures of isolated myocytes.

Key words

Na+/H+ exchanger (NHE-1)membranecDNA(Human heart)

Copyright information

© Kluwer Academic Publshers 1993