Molecular and Cellular Biochemistry

, Volume 145, Issue 2, pp 185–187

Rapid isolation of any known genes from whole cells of yeast by PCR

  • Dindial Ramotar
Short Communication

DOI: 10.1007/BF00935491

Cite this article as:
Ramotar, D. Mol Cell Biochem (1995) 145: 185. doi:10.1007/BF00935491


A short protocol was developed that allows the rapid isolation of any knownSaccharomyces cerevisiae gene. Two known genes,APN1 andIMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.

Key words

yeastpolymerase chain reactiongenemethod

Copyright information

© Kluwer Academic Publishers 1995

Authors and Affiliations

  • Dindial Ramotar
    • 1
  1. 1.Environment and HealthCHUL Research CentreSte-FoyCanada