, Volume 145, Issue 2, pp 185-187

Rapid isolation of any known genes from whole cells of yeast by PCR

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Abstract

A short protocol was developed that allows the rapid isolation of any knownSaccharomyces cerevisiae gene. Two known genes,APN1 andIMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.