Parasitology Research

, Volume 81, Issue 6, pp 465–469

Ultrastructure ofBlastocystis hominis cysts

Authors

  • V. Zaman
    • Department of MicrobiologyAga Khan University
  • J. Howe
    • Department of MicrobiologyNational University of Singapore
  • M. Ng
    • Department of MicrobiologyNational University of Singapore
Original Paper

DOI: 10.1007/BF00931787

Cite this article as:
Zaman, V., Howe, J. & Ng, M. Parasitol Res (1995) 81: 465. doi:10.1007/BF00931787

Abstract

Blastocystis hominis cysts were concentrated from human faeces by repeated washing in distilled water and centrifugation on Ficoll-Paque. This procedure gave a concentrated suspension of cysts without yielding any non-cystic forms. The cysts were examined by both transmission (TEM) and scanning electron microscopy (SEM). Cysts were surrounded by a fibrillar layer that appeared porous in SEM. Many naked cysts without the fibrillar layer were also visible in SEM. They were variable in shape but mostly circular to oval. The diameter of the cyst without the fibrillar layer was 3.5 μm in both TEM and SEM. The nuclear structure was typical ofBlastocystis and exhibited multiple mitochondria with poorly developed cristae. Glycogen was present in small to large clumps in the cytoplasm. The cyst wall was 5–100 nm thick and was bounded internally by an electron-dense plasmalemma.

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© Springer-Verlag 1995