Parasitology Research

, Volume 75, Issue 8, pp 599–603

Tissue cyst rupture in mice chronically infected withToxoplasma gondii

An immunocytochemical and ultrastructural study
  • D. J. P. Ferguson
  • W. M. Hutchison
  • E. Pettersen
Original Investigations

DOI: 10.1007/BF00930955

Cite this article as:
Ferguson, D.J.P., Hutchison, W.M. & Pettersen, E. Parasitol Res (1989) 75: 599. doi:10.1007/BF00930955

Abstract

The incidence and effect of tissue cyst rupture in the brains of mice chronically infected withToxoplasma gondii was studied by immunocytochemistry and electron microscopy. Cyst rupture was extremely rare (2 of 750 tissue cysts) irrespective of the interval post-infection. The event was associated with a rapid cell-mediated immune response, giving rise to microglial or inflammatory nodules. Macrophages were observed to engulf and degrade the cystozoites and cyst debris. Initially, the nodules contained large amounts of immunologically reactive material, but this was degraded with the majority (94%) of lesions containing no recognizable parasites orToxoplasma antigens. There was little evidence of parasite multiplication or new cyst formation associated with cyst rupture. This study shows that although intermittent cyst rupture occurs, in immunocompetent individuals the immune response limits the potential damage from the release of large numbers of infective organisms to small microglial/inflammatory nodules.

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • D. J. P. Ferguson
    • 1
  • W. M. Hutchison
    • 2
  • E. Pettersen
    • 3
  1. 1.Nuffield Department of PathologyUniversity of Oxford, John Radcliffe HospitalOxfordUK
  2. 2.Biology Division, Department of Bioscience and BiotechnologyUniversity of StrathclydeGlasgowUK
  3. 3.Fao/Who Collaborating Centre for Research and Reference in ToxoplasmosisStatens SeruminstitutCopenhagenDenmark

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