Parasitology Research

, Volume 77, Issue 8, pp 697–702

Application of the Western blotting procedure for the immunodiagnosis of human toxocariasis

  • J. -F. Magnaval
  • R. Fabre
  • P. Maurières
  • J. -P. Charlet
  • B. de Larrard
Original Investigations

DOI: 10.1007/BF00928685

Cite this article as:
Magnaval, J.F., Fabre, R., Maurières, P. et al. Parasitol Res (1991) 77: 697. doi:10.1007/BF00928685

Abstract

To improve the immunodiagnosis of human toxocaral disease, a sensitive and specific assay using the Western blotting procedure (WB) with excretory-secretory antigens fromToxocara canis larvae (TES) was developed and compared with the standard enzymelinked immunosorbent assay method (TES-ELISA) using the same antigens. We tested groups of sera from laboratory animals or patients presenting with toxocariasis or other helminthic diseases and a group of sera from people dwelling in an area endemic for toxocariasis who exhibited hypereosinophilia. Statistically, the WB assay correlated well with TES-ELISA, but the former was more specific for banding patterns corresponding to low-molecular-weight fractions, thus avoiding problems of cross-reactivity with sera infected with other helminthic diseases.

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • J. -F. Magnaval
    • 1
  • R. Fabre
    • 1
  • P. Maurières
    • 1
  • J. -P. Charlet
    • 1
  • B. de Larrard
    • 1
  1. 1.Laboratoire de ParasitologieCHU Toulouse PurpanToulouseFrance