Original Investigations

Zeitschrift für Parasitenkunde

, Volume 72, Issue 1, pp 97-104

First online:

Collagen immunotyping of the hepatic fibrosis in human alveolar echinococcosis

  • Dominique A. VuittonAffiliated withServices d'Hépatologie, de Chirurgie Viscérale et d'Anatomie Pathologique, Centre Hospitalier Universitaire
  • , Sylviane Guerret-StockerAffiliated withLaboratoire de Pathologie Cellulaire du Foie, CNRS, ERA 819, Institut Pasteur
  • , Jean-Pierre CarbilletAffiliated withServices d'Hépatologie, de Chirurgie Viscérale et d'Anatomie Pathologique, Centre Hospitalier Universitaire
  • , Georges MantionAffiliated withServices d'Hépatologie, de Chirurgie Viscérale et d'Anatomie Pathologique, Centre Hospitalier Universitaire
  • , Jean-Philippe MiguetAffiliated withServices d'Hépatologie, de Chirurgie Viscérale et d'Anatomie Pathologique, Centre Hospitalier Universitaire
  • , Jean-Alexis GrimaudAffiliated withLaboratoire de Pathologie Cellulaire du Foie, CNRS, ERA 819, Institut Pasteur

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Abstract

An extremely dense fibrosis is responsible for the main complications of alveolar echinococcosis (AE), a severe parasitic disease due to the development in the liver of a larval form of the cestodeEchinococcus multilocularis. Immunotyping of collagens present in fibrous, granulomatous, and normal areas of the liver was performed in nine patients with AE. Immunofluorescent labelling of collagen types showed that dense fibrosis was entirely composed of thick bundles of type I and type III collagens, surrounding old parasitic vesicles with an autofluorescent laminated layer. In granulomatous areas, collagens were labelled as clusters consisting mainly of type III procollagen and collagen. Concentric bundles of type III collagen surrounded active parasitic vesicles. An enhancement of the normal labelling in portal spaces of the intact liver was present in all the patients. These results suggest that irreversible acellular keloid scar-like fibrosis observed in AE is the ultimate result of cytotoxic and fibrogenetic events related to the immune response of the host which are taking place initially in the granulomatous area surrounding the young parasite larvae.