Molecular and Cellular Biochemistry

, Volume 125, Issue 1, pp 11–18

Acute treatment with tumour necrosis factor-α induces changes in protein metabolism in rat skeletal muscle

  • Cèlia García-Martínez
  • Francisco J. López-Soriano
  • Josep M. Argilés
Article

DOI: 10.1007/BF00926829

Cite this article as:
García-Martínez, C., López-Soriano, F.J. & Argilés, J.M. Mol Cell Biochem (1993) 125: 11. doi:10.1007/BF00926829

Abstract

Acute treatment of rats with recombinant tumour necrosis factor (TNF-α) caused an enhanced proteolytic rate —measured as tyrosine released in the presence of cycloheximide — insoleus muscle (34%). The cytokine treatment also decreased the rate of protein synthesis in this muscle (22%) while it had no effect upon the same parameter inextensor digitorum longus (EDL) (26%) muscle. In addition, treatment of rats with TNF-α increased amino acid uptake by transport system A in the incubated muscles both insoleus (45%) andEDL (99%) in the presence of insulin in the incubating medium. This effect was not associated with a direct action of TNF on muscle since the addition of different concentrations of the cytokine to the preparations did not alter the uptake of α-(methyl)-aminoisobutyric acid by the incubated muscles. It can be concluded that acute TNF-α treatment causes changes in protein metabolism in red-type muscles — suchsoleus — while little effects are seen in white-type muscles — such as EDL. The results presented may, to some extent, be related to the cachectic response associated with cancer and inflammation.

Key Words

tumor necrosis factorcytokinesprotein metabolismskeletal muscle

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Cèlia García-Martínez
    • 1
  • Francisco J. López-Soriano
    • 1
  • Josep M. Argilés
    • 1
  1. 1.Unitat de Bioquímica B, Departament de Bioquímica i Fisiologia, Facultat de BiologiaUniversitat de BarcelonaBarcelonaSpain