International Ophthalmology

, Volume 16, Issue 3, pp 167–176

Biophysical characteristics of the Meibomian lipid layer underin vitro conditions

  • Thomas Kaercher
  • Dietmar Möbius
  • Rüdiger Welt
Original Papers

DOI: 10.1007/BF00916437

Cite this article as:
Kaercher, T., Möbius, D. & Welt, R. Int Ophthalmol (1992) 16: 167. doi:10.1007/BF00916437

Abstract

The biophysical behaviour of the Meibomian gland secretion was tested underin vitro conditions. Thereby, simultaneous recording of surface pressure and surface potential was performed. The Meibomian lipid layer was compared with other surface-active components like polyvinylalcohol and polyvinylpyrrolidone. On the other hand, Eledoisin was tested as an example for a surface-inactive substance. An attempt was made to describe the biophysical interaction between a given artificial tear substitute and the Meibomian lipid layer. With respect to the surface potential Dipalmitoyl-phosphatidyl-choline was established as an analogue for Meibomian gland secretion. Fluorescence measurements in the presence of a cyanine dye (1 N,N′ dioctadecyloxacarbocyanine) were used as a method to localize the site of the characteristic potential change. From the fluorescence spectra under compression we conclude that the molecular change takes place at the lipid-subphase interface of the Meibomian lipid layer.

Key words

Meibomian gland secretionsurface potentialsurface pressure

Abbreviations

DPPC

dipalmitoyl-phosphatidyl-choline

MGS

Meibomian gland secretion

PVA

polyvinyl-alcohol

PVP

polyvinyl-pyrrolidone

Copyright information

© Kluwer Academic Publishers 1992

Authors and Affiliations

  • Thomas Kaercher
    • 1
  • Dietmar Möbius
    • 2
  • Rüdiger Welt
    • 1
  1. 1.Augenklinik LudwigshafenLudwigshafenGermany
  2. 2.Max-Planck-Institut für Biophysikalische ChemieArbeitsgruppe Molekular organisierte SystemeGöttingen-NikolausbergGermany