Glutathione in bronchoalveolar lavage fluid from smokers is related to humoral markers of inflammatory cell activity
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
Cigarette smoking results in variable degrees of inflammation in the lower respiratory tract. Furthermore, smoking produces oxidant-mediated changes in the lung, important to the pathogenesis of emphysema. Since glutathione can neutralize reactive oxygen species and prevent peroxidation of unsaturated lipids, it may constitute an important component of the lung's defense against oxidant and inflammatory injury. In the present study, broncholaveolar lavage (BAL) was performed in 27 smokers, and the concentrations of total glutathione as well as the cellular and humoral markers of inflammatory activity were studied. There were significant correlations between total glutathione and neutrophils; two neutrophil granule components, myeloperoxidase and elastase; and chemotactic activity for neutrophils. Moreover, the total glutathione correlated with the eosinophil cationic protein (ECP), a granule constituent of the eosinophil, with two locally produced antiproteases, secretory leukocyte protease inhibitor (SLPI) and antichymotrypsin (ACHY), but not with an α1-protease inhibitor and albumin. These data suggest that the total glutathione levels in BAL fluid may reflect a degree of oxidative and inflammatory stress caused by cigarette smoke, and they are therefore likely to contribute to the protection against this stress.
- Hunninghake, G. W., J. E. Gadek, O. Kawanami, V. J. Ferrans, andR. G. Crystal. 1979. Inflammatory and immune processes in the human lung in health and disease: Evaluation by bronchoalveolar lavage.Am. J. Pathol. 97:149–206.
- Hunninghake, G. W. andR. G. Crystal. 1983. Cigarette smoking and lung destruction. Accumulation of neutrophils in the lungs of cigarette smokers.Am. Rev. Respir. Dis. 128:833–838.
- Babior, B. M. 1978. Oxygen-dependent microbial killing by phagocytes.N. Engl. J. Med. 298:659–668, 721–725.
- Klebanoff, S. J. 1967. A perioxidase-mediated antimicrobial system in leukocytes.J. Clin. Invest. 46:1078.
- Janoff, A., H. Carp, P. Laurent, andL. Raju. 1983. The role of oxidative process in emphysema.Am. Rev. Respir. Dis. 127:831–838.
- Cantin, A. M., S. L. North, R. C. Hubbard, andR. G. Crystal. 1987. Normal alveolar epithelial fluid contains high levels of glutathione.J. Appl. Physiol. 63:152–157.
- Fryksmark, U., K. Ohlsson, A. Polling, andH. Tegner. 1982. Distribution of antileukoprotease in upper respiratory mucosa.Ann. Otol. Rhinol. Laryngol. 91:268–271.
- Ryley, H. C., andJ. D. Brogan. 1973. Quantitative immunoelectrophoretic analysis of the plasma proteins in the sol phase of sputum from patients with chronic bronchitis.J. Clin. Pathol. 26:852–856.
- Venge, P., L.-E. Roxin, andI. Olsson. 1977. Radioimmunoassay of human eosinophil cationic protein.Br. J. Haematol. 37:331–335.
- Venge, P., R. Hällgren, G. Stålenheim, andI. Olsson. 1979. Effect of serum and cations on the selective release of granular proteins from human neutrophils during phagocytosis.Scand. J. Haematol. 22:317–326.
- Olofsson, T., I. Olsson, P. Venge, andB. Elgefors. 1977. Serum myeloperoxidase and lactoferrin in neutropenia.Scand. J. Haematol. 18:73–80.
- Fryksmark, U., K. Ohlsson, M. Rosengren, andH. Tegner. 1981. A radioimmunoassay for measurement and characterization of human antileukoprotease in serum.Hoppe-Seyler's Z. Physiol. Chem. 362:1273–1277.
- Ohlsson, K., M. Rosengren, G. Stetler, M. Brewer, K. K. Hole, andR. C. Thompson. 1987. Structure, genomic organization and tissue distribution of human secretory leucocyte protease inhibitor (SLPI): A potent inhibitor of neutrophil elastase.In: Pulmonary Emphysema and Proteolysis, Vol. II. J. G. Taylor, and C. Mittman, editors. Academic Press, New York, 307–324.
- Ohlsson, K., andA.-S. Olsson. 1978. Immunoreactive granulocyte elastase in human serum.Hoppe-Seyler's Z. Physiol. Chem. 359:1531–1539.
- Laurell, C.-B. 1972. Electroimmunoassay.Scand. J. Clin. Lab. Invest. 29(suppl):21–37.
- Håkansson, L., D. Westerlund, andP. Venge. 1987. A new method for measurement of eosinophil migration.J. Leukocyte. Biol. 42:689–696.
- Wieslander, E., M. Linden, L. Håkansson, A. Eklund, E. Blaschke, R. Brattsand, andP. Venge. 1987. Human alveolar macrophages from smokers have an impaired capacity to secrete LTB4 but not other chemotactic factors.Eur. J. Respir. Dis. 71:263–272.
- Cotgreave, I., andP. Moldéus. 1986. Methodologies for the application of monobromobimaneto the simultaneous analysis of soluble and protein thiol components of biological systems.J. Biochem. Biophys. Methods 13:231–249.
- Andersson, M. E. 1985. Determination of gluthatione disulfide in biological samples.Methods Enzymol. 113:548–555.
- Rennard, S. I., G. Basset, D. Lecossier, K. M. O'Donnell, P. G. Martin andR. G. Crystal. 1986. Estimation or absolute volume of epithelial lining fluid recovered by bronchoalveolar lavage using urea as an endogenous marker of dilution.J. Appl. Physiol. 60:532–538.
- Ohlsson, K., andU. Åkesson. 1976. α1-Antichymotrypsin interaction with cationic proteases from granulocytes.Clin. Chim. Acta 73:285–289.
- Jenkinson, S. G., R. D. Black, andR. A. Lawrence. 1988. Glutathione concentrations in rat lung bronchoalveolar lavage fluid: Effects of hyperoxia.J. Lab. Clin. Med. 112:345–351.
- Glutathione in bronchoalveolar lavage fluid from smokers is related to humoral markers of inflammatory cell activity
Volume 13, Issue 6 , pp 651-658
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- Industry Sectors
- Author Affiliations
- 1. Department of Clinical Research, AB Draco, Lund, Sweden
- 2. Department of Clinical Chemistry, University Hospital, Uppsala, Sweden
- 3. Department of Surgical Pathophysiology, University of Lund, Malmö, Sweden
- 4. Department of Pharmacokinetics, Research and Development Laboratories, AB Draco, Lund, Sweden
- 5. Department of Otorhinolaryngology, University of Lund Malmö General Hospital, Malmö, Sweden