Article

Antonie van Leeuwenhoek

, Volume 64, Issue 3, pp 253-260

First online:

Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test

Proposal for the creation of a new genus Paenibacillus
  • Carol AshAffiliated withDepartment of Microbiology, Agricultural and Food Research Council, Institute of Food Research
  • , Fergus G. PriestAffiliated withDepartment of Biological Sciences, Heriot-Watt University
  • , M. David CollinsAffiliated withDepartment of Microbiology, Agricultural and Food Research Council, Institute of Food Research

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Abstract

Comparative 16S rRNA sequence analysis has demonstrated that the genusBacillus consists of at least five phyletic lines. rRNA group 3 bacilli of Ash, Farrow, Wallbanks and Collins (1991) comprisingBacillus polymyxa and close relatives is phylogenetically so removed fromBacillus subtilis, the type species of the genus and other aerobic, endospore-forming bacilli that they warrant reclassification in a new genusPaenibacillus. The genusPaenibacillus can be readily distinguished from otherBacillus groups using a battery of phenotypic characters and a highly specific gene probe based on 16S rRNA.

Key words

Bacillus Paenibacillus rRNA probe