Wilhelm Roux's archives of developmental biology

, Volume 185, Issue 4, pp 363–370

Fusion ofDrosophila eye-antennal imaginal discs during differentiation in vitro

  • Martin J. Milner
  • John L. Haynie

DOI: 10.1007/BF00848522

Cite this article as:
Milner, M.J. & Haynie, J.L. Wilhelm Roux' Archiv (1979) 185: 363. doi:10.1007/BF00848522


Pairs of eye-antennal discs, attached to the cephalic ganglia, were cultured in vitro with a concentration of β-ecdysone optimal for imaginal differentiation. The eye-antennal discs fused to form a vesicle inside which the antennae were partially everted, and on the inner surface of which imaginal structures differentiated. The epithelium of the discs was continuous, and an integrated pattern of bristles and hairs differentiated in vitro. In particular, the median ocellus, a unified structure derived partially from each disc, differentiated normally.

Key words

DrosophilaImaginal discsPattern formationβ-ecdysoneTissue culture

Copyright information

© Springer-Verlag 1979

Authors and Affiliations

  • Martin J. Milner
    • 1
  • John L. Haynie
    • 1
  1. 1.Department of ZoologieUniversity of WashingtonSeattleUSA
  2. 2.Zoologisches InstitutUniversität ZürichZürichSwitzerland