Journal of Wood Science

, Volume 44, Issue 6, pp 463–468

In vitro screening of angiotensin I-converting enzyme inhibitors from Japanese cedar (Cryptomeria japonica)


    • Faculty of AgricultureShizuoka University
  • Ayako Shimada
    • Faculty of AgricultureShizuoka University
  • Akiko Miyano
    • Faculty of AgricultureShizuoka University
  • Tomoaki Nishida
    • Faculty of AgricultureShizuoka University
  • Tohru Mitsunaga
    • Faculty of BioresourcesMie University
Original Article

DOI: 10.1007/BF00833411

Cite this article as:
Tsutsumi, Y., Shimada, A., Miyano, A. et al. J Wood Sci (1998) 44: 463. doi:10.1007/BF00833411


Screening and isolation of angiotensin I-converting enzyme (ACE) inhibitors from Japanese cedar (Cryptomeria japonica) based on the in vitro ACE inhibitory assay were attempted. The ethanol extract from outer bark showed the highest inhibitory activity (IC50 is 16μg/ml) among 24 extracts prepared from roots, leaves, heartwood, sapwood, inner bark, and outer bark by successive extraction with four solvents. The fractionation of the outer bark ethanol extract followed by the bioassay resulted in the isolation of two strong ACE inhibitors, catechin and dimeric procyanidin B3. The bioassay of three flavan-3-ols including (+)-catechin and six flavones revealed that most of these compounds have high ACE inhibitory activity. The results suggest that the phenolic hydroxyl group at the C7 position and heterocyclic oxygen atom of these compounds are important for expressing the inhibitory activity.

Key words

ACE inhibitorCryptomeria japonicaCatechinProcyanidin B3Flavonoid

Copyright information

© The Japan Wood Research Society 1998