Experimental Biology

Bulletin of Experimental Biology and Medicine

, Volume 57, Issue 1, pp 94-98

First online:

Cytophysiological differences between the embryoblast and trophoblast of rat embryos as revealed by vital staining

  • N. A. SamoshkinaAffiliated withInstitute of Experimental Medicine of the AMN SSSR

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The cytophysiological method was used to study the early stages of rat development in vitro. Along with the formation of granules de novo in the cytoplasm of embryonic cells neutral red stained some pre-existing structures. The type of intravital staining and the intensity of granule formation differed at various developmental stages. In the early preimplantation stages (from the second to the fifth day) the intensity of granule formation in the embryoblast cells was greater than in the trophoblast cells. Conversely, immediately after the implantation (the seventh-tenth days) the extraembryonic formations (yolk entoderm, ectoplacental cone, giant cells of the trophoblast) stain more intensely; embryonic and intestinal entoderm stain much more weakly. The character of granule dis tribution in the tissues of rat embryos at various developmental periods is explained by the peculiarities of growth and differentiation of individual anlagen of mammals at various stages of ontogenesis.