Inhibition of the mitochondrial calcium uniporter by antibodies against a 40-kDa glycorproteinT
- Cite this article as:
- Saris, NE.L., Sirota, T.V., Virtanen, I. et al. J Bioenerg Biomembr (1993) 25: 307. doi:10.1007/BF00762591
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Polyclonal rabbit antibodies against a Ca2+-binding mitochondrial glycoprotein were found to inhibit the uniporter-mediated transport of Ca2+ in mitoplasts prepared from rat liver mitochondria. Spermine, a modulator of the uniporter, decreased the inhibition. This glycoprotein ofMr 40,000, isolated from beef heart mitochondria and earlier shown to form Ca2+-conducting channels in black-lipid membranes, thus is a good candidate for being a component of the uniporter. Antibody-IgG was found to specifically bind to mitochondria in human fibroblasts.