Cells and bubbles in sparged bioreactors
- Jeffrey J. Chalmers
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Ever since animal cells have been grownin-vitro, various techniques have been used to supply the cells with oxygen. The most simple and commonly used ‘large-scale’ technique to provide oxygen is through the introduction of gas bubbles. However, almost since the beginning ofin-vitro cell culture, empirical observations have indicated that bubbles can be detrimental to the cells. This review will discuss the background of the problem, review the relevant research on the topic, attempt to provide a coherent summary of what we know from all of this research, and finally outline what still needs to be investigated. Specific topics to be covered include: experimental correlations of cell damage with bubbles, cell attachment to bubbles, the hydrodynamics of bubble repture, bioreactor studies, visualization studies, and computer simulations and qualification of cell death as a result of bubble rupture.
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- Augenstein DC, Sinskey AJ, Wang DIC (1971) Effect of shear on the death of two strains of mammalian tissue cells. Biotechnol. Bioeng. 13: 409–418.
- Aunins JG, Croughan MS, Wang DIC (1986) Engineering developments in homogenous culture of animal cells: Oxygenation of reactors and scaleup. Biotechnol. Bioeng. 17: 399–723.
- Backer MP, Metzger LS, Slaber PL, Nevitt KL, Boder GB (1988) Large-scale production of nonoclonal antibodies in suspension culture. Biotechnol. Bioeng. 32: 993–1000.
- Bavarian F, Fan LS, Chalmers JJ (1991) Microscopic visualization of insect cell-bubble interactions. I: Rising bubbles, air-medium interface, and the foam layer. Biotechnol. Prog. 7: 140–150.
- Boulton-Stone JM, Blake JR (1993) Gas-bubbles bursting at a free surface. J. Fluid Mech. 154: 437–466.
- Cherry RS, Papoutsakis ET (1986) Hydrodynamic effects on cells in agitated tissue culture reactors. Bioproc. Eng. 1: 29–41.
- Chalmers JJ, Bavarian F (2991) Microscopic visualization of insect cell-bubble interactions. II: The bubble film and bubble rupture. Biotechnol. Prog. 7: 151–158.
- Croughan MS, Hamel JF, Wang DIC (1987) Hydrodynamic effects in animal cells grown in microcarrier cultures. Biotechnol. Bioeng. 29: 130–141.
- Dodge TC, Hu WS (1986) Growth of hybridoma cells under different agiatation conditions. Biotechnol. Letters 8: 683–686.
- Garcia-Briones MA, Brodkey RS, Chalmers JJ (1994) Computer simulations of the rupture of a gas bubble at a gas-liquid interface and its implications in animal cell damage. Chem. Eng. Sci. 49: 2301–2320.
- Garcia-Briones MA, Chalmers JJ (1992) Cell-bubble interactions: Mechanims of suspended cell damage. Ann. N.Y, Acad. Sci. 665: 219–229.
- Goldblum S, Bae Y, Hink WF, Chalmers JJ (1990) Protective effect of methylcellulose and other polymers on insect cells subjected to laminar shear stress. Biotechnol. Prog. 6: 383–390.
- Handa-Corrigan A, Emary AN, Spier RE (1989) Effect of gas-liquid interfaces on the growth of suspended mammalian cells: mechanisms of cell damage by bubbles. Enzyme Microb. Technol. 11: 230–235.
- Handa A, Emary AN, Spier RE (1987) On the evaluation of gasliquid interfacial effects on hydridoma viability in bubble column bioreactors. Dev. Biol. Stand. 66: 241–253.
- Hu WS, Meier J, Wang DIC (1985) A mechanistic analysis of the inoculum requirement for the cultivation of mammalian cells on microcarriers. Biotechnol. Bioeng. 27: 585–595.
- Jobses I, Martens D, Tramper J (1991) Lethal events during gas sparging in animal cell culture. Biotech. Bioeng. 37: 484–490.
- Kilburn DG, Webb FC (1968) The cultivation of animal cells at controlled dissolved oxygen partial pressure. Biotechnol. Bioeng. 10: 801–814.
- Kunas KT, Papoutsakis ET (1990a) Damage mechanisms of suspended animal cells in agitated bioreactors with and without bubble entrainment. Biotechnol. Bioeng. 36: 476–483.
- Kunas KT, Papoutsakis ET (1990b) The protective effect of serum against hydrodynamic damage of hydridoma cells in agitated and surface-areated bioreactors. J. Biotechnol. 15: 57–70.
- Lee GM, Huard TK, Kaminski MS, Palsson BO (1988) Effect of mechanical agitation on hydridoma cell growth. Biotechnol. Letters 10: 625–628.
- MacIntyre F (1972) Flow patterns in breaking bubbles. J Geophys. Res. 77: 5211–5228.
- MacIntyre F (1968) Bubbles: a boundary-layer ‘microtom” for micron-thick samples of a liquid surface. J. Phys. Chem. 72: 589–592.
- Martens DE, de Gooijer CD, Beuvery EC, Tramper J (1992) Effect of serum concentration on hybridoma viable cell density and production of monoclonal antibodies in CSTRs and on shear sensitivity in air-lift loop reactors. Biotechnol. Bioeng. 39: 891–897.
- Murhammer DW, Goochee CF (1990) Sparged animal cell bioreactors: mechanism of cell damage and Pluronic F-68 protection. Biotechnol. Prog. 6: 391–397.
- Oh SKW, Nienow AW, Al-Rubeai M, Emary AN (1989) The effect of agiatation intensity with and without continuous sparging on the growth and antibody production of hybridoma cells. J. Biotechnol. 12: 45–62.
- Orton D, Wang DIC (1991) Fluorescent Visualization of Cell Death in Bubble Areated Bioreactors. Cell Culture Engineering III, Engineering Foundation, Feb. 2–7,
- Ruyan WS, Gyer RP (1963) Growth of L cell suspensions on a Warburg apparatus. Proc. Soc. Bio. Med. 103: 252–254.
- Schurch U, Kramer H, Einsle A, Widmer F, Eppenberger HM (1988) Experimental evaluation of laminar shear stress on the behaviour of hybridoma mass cell cultures producing monoclonal antibodies against mitochondrial creatine kinase. J. Biotechnol. 7: 179–184.
- Sinskey AJ, Fleischaker RJ, Tyo MA, Giard DJ, Wang DIC (1981) Production of cell derived products: virus and interferon. Ann. N.Y. Acad. Sci. 369: 47–59.
- Smith CG, Greenfield PF, Randerson DH (1987) A technique for determining the shear sensitivity of mammalian cells in suspension culture. Biotechnol. Techn. 1: 39–44.
- Swim HE, Parker RF (1960) Effect of Pluronic F-68 on growth of fibroblasts in suspension on rotary shakers. Proc. Soc. Biol. Med. 103: 252–254.
- Tramper J, Smit JD, Straatman J, Valk JM (1988) Bubble-column design for growth of fragile insect cells. Bioprocess Engin. 3: 37–41.
- Tramper J, Williams JB, Joustra D (1986) Shear sensitivity of insect cells in suspension. Enzyme Microb. Technol. 8: 33–36.
- Trinh K, Garcia-Briones MA, Hink FH, Chalmers JJ (1994) Quantification of damage to suspended insect cells as a result of bubble rupture. Biotechnol. Bioeng. 43: 37–45.
- Wang NS, Yang JD, Calabrese RV, Chang KC (1994) Unified modeling framework of cell death due to bubbles in agitated and sparged bioreactors. J. Biotechnol. 33: 107–122.
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- Cells and bubbles in sparged bioreactors
Volume 15, Issue 1-3 , pp 311-320
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- 1. Department of Chemical Engineering, Ohio State University, 140 W 19th Ave., 43210, Columbus, OH, USA