, Volume 13, Issue 3, pp 149–159

Perfusion culture apparatus for suspended mammalian cells

  • Michiyuki Tokashiki
  • Hiroyuki Takamatsu
Original Research Papers

DOI: 10.1007/BF00749811

Cite this article as:
Tokashiki, M. & Takamatsu, H. Cytotechnology (1993) 13: 149. doi:10.1007/BF00749811


A variety of processes have been proposed for mammalian cell culture in the commercial production of useful substances (e.g., monoclonal antibodies, therapeutic and diagnostics proteins). Among them, the perfusion culture of suspended non-immobilized cells is the most advantageous. Perfusion culture can be classified by the separation process of suspended cells from the culture mixture into three types, namely filtration, gravitational settling and centrifugation. From a commercial point of view, the present situation and technical problems of suspended-cell perfusion culture will be reviewed based on the three types, The recent development of perfusion culture has been carried out mainly on the filtration separation process, but the centrifugation process seems to have a promising future because of operation stability and scale-up feasibility. The reasons will be explained in details.

Key words

anchorage independent mammalian cells centrifugation filtration gravitational settling high density perfusion culture suspended-cell culture 

Copyright information

© Kluwer Academic Publishers 1993

Authors and Affiliations

  • Michiyuki Tokashiki
    • 1
  • Hiroyuki Takamatsu
    • 1
  1. 1.Tokyo Research CenterTeijin LimitedHino-shi, TokyoJapan

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