Research Papers

Journal of Bioenergetics and Biomembranes

, Volume 13, Issue 5, pp 241-253

First online:

Generation of hydrogen peroxide on oxidation of NADH by hepatic plasma membranes

  • T. RamasarmaAffiliated withDepartment of Biochemistry, Indian Institute of Science
  • , Anand SwaroopAffiliated withDepartment of Biochemistry, Indian Institute of Science
  • , W. MacKellarAffiliated withDepartment of Biological Sciences, Purdue University
  • , F. L. CraneAffiliated withDepartment of Biological Sciences, Purdue University

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access


The oxidation of NADH by mouse liver plasma membranes was shown to be accompanied by the formation of H2O2. The rate of H2O2 formation was less than one-tenth the rate of oxygen uptake and much slower than the rate of reduction of artificial electron acceptors. The optimum pH for this reaction was 7.0 and theK m value for NADH was found to be 3×10−6 M. The H2O2-generating system of plasma membranes was inhibited by quinacrine and azide, thus distinguishing it from similar activities in endoplasmic reticulum and mitochondria. Both NADH and NADPH served as substrates for plasma membrane H2O2 generation. Superoxide dismutase and adriamycin inhibited the reaction. Vanadate, known to stimulate the oxidation of NADH by plasma membranes, did not increase the formation of H2O2. In view of the growing evidence that H2O2 can be involved in metabolic control, the formation of H2O2 by a plasma membrane NAD(P)H oxidase system may be pertinent to control sites at the plasma membrane.

Key Words

Plasma membrane NADH oxidase plasma membrane hydrogen peroxide formation hydrogen peroxide formation by NADH oxidation plasma membrane redox quinacrine inhibition plasma membrane NADPH oxidation-plasma membrane