Histochemistry

, Volume 95, Issue 1, pp 43–54

Estradiol distribution and penetration in rat skin after topical application, studied by high resolution autoradiography

  • H. J. Bidmon
  • J. D. Pitts
  • H. F. Solomon
  • J. V. Bondi
  • W. E. Stumpf
Article

DOI: 10.1007/BF00737227

Cite this article as:
Bidmon, H.J., Pitts, J.D., Solomon, H.F. et al. Histochemistry (1990) 95: 43. doi:10.1007/BF00737227

Summary

Transdermal pathways and targets in the skin for estradiol were investigated using dry-mount autoradiography.3H-estradiol-17β was applied at doses of 30.1 pmol, 120.4 pmol and 301 pmol/cm2 to shaved rat skin in the dorsal neck region. Vehicles were DMSO, ethylene glycol or sesame oil. After 2 h of topical treatment with 30.1 pmol3H-estradiol×cm−2 dissolved in DMSO a distinct cellular distribution was apparent. Target cells with concentrations of radioactivity were found in epidermis, sebaceous glands, dermal papillae of hair and fibroblasts. After treatment with 120.4 and 301 pmol/cm2, a penetration gradient of radioactivity was recognizable however it masked specific cellular and subcellular uptake. The stratum corneum accumulated and retained radioactivity, apparently forming a depot for the hormone. Strong concentration and retention of the hormone was conspicious in sebaceous glands for more than 24 h, suggesting that sebaceous glands serve as a second storage site for the hormone. In all autoradiograms two penetration pathways to the dermis were visible: one through the stratum corneum and epidermis, the other through the hair canals and hair sheaths.

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • H. J. Bidmon
    • 1
  • J. D. Pitts
    • 1
  • H. F. Solomon
    • 1
    • 2
  • J. V. Bondi
    • 2
  • W. E. Stumpf
    • 1
  1. 1.Departments of Cell Biology and Anatomy, and PharmacologyUniversity of North CarolinaChapel Hill
  2. 2.Merck Sharp & Dohme Research LaboratoriesWest PointUSA
  3. 3.R.W. Johnson Pharmaceutical Research InstituteSpring HouseUSA