Surgery Today

, Volume 23, Issue 6, pp 491–495

Mucin-producing pancreatic tumors: A study of nuclear DNA content by flow cytometry

  • Yoshiaki Murakami
  • Takashi Yokoyama
  • Takashi Kodama
  • Yoshio Takesue
  • Mitsuaki Okita
  • Atsushi Nakamitsu
  • Yuji Imamura
  • Takahiro Santo
  • Hiroaki Tsumura
  • Katsunari Miyamoto
  • Yuichiro Matsuura
Original Articles

DOI: 10.1007/BF00730622

Cite this article as:
Murakami, Y., Yokoyama, T., Kodama, T. et al. Surg Today (1993) 23: 491. doi:10.1007/BF00730622

Abstract

Nuclear DNA content in eight surgically resected mucin-producing pancreatic tumors (MPPT) consisting of two mucinous intraductal adenocarcinomas (MIDAC), two mucinous intraductal adenomas (MIDA), one mucinous cystadenocarcinoma (MCAC), and three mucinous cystadenomas (MCA) were measured by flow cytometry using paraffin-embedded tissue samples. The technique of Shutte was used for the preparation of paraffin-embedded tissue into single dissociated nuclei, while the method of Vindelov was used for staining the isolated nuclei with propidium iodine. Clinicopathologically, the four patients with MIDAC or MIDA were all male and had cystic lesions with a dilated pancreatic duct at the head of the pancreas, while the four patients with MCAC or MCA were all females and had cystic tumors at either the body or tail of the pancreas. All eight patients with MPPT had no metastasis to the regional lymph nodes and were all still alive without recurrence. In an analysis of nuclear DNA content, seven of eight patients had DNA diploid tumors while one patient with a MIDAC perforating the duodenum and choledochus had a DNA aneuploid tumor. Thus, these findings suggest that DNA diploid patterns in MPPT might be associated with a favorable prognosis in MPPT although some patients whose MPPT invaded the surrounding organs might have DNA aneuploid tumors.

Key Words

mucin-producing pancreatic tumornuclear DNA contentdiploidaneuploidflow cytometryprognosis

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Yoshiaki Murakami
    • 1
  • Takashi Yokoyama
    • 2
  • Takashi Kodama
    • 1
  • Yoshio Takesue
    • 1
  • Mitsuaki Okita
    • 1
  • Atsushi Nakamitsu
    • 1
  • Yuji Imamura
    • 1
  • Takahiro Santo
    • 1
  • Hiroaki Tsumura
    • 1
  • Katsunari Miyamoto
    • 1
  • Yuichiro Matsuura
    • 1
  1. 1.First Department of SurgeryHiroshima University School of MedicineHiroshimaJapan
  2. 2.Department of General MedicineHiroshima University School of MedicineHiroshimaJapan