Journal of Muscle Research & Cell Motility

, Volume 4, Issue 1, pp 83–93

The effect of cytochalasin and glutaraldehyde on F-actin filaments containing muscle and non-muscle tropomyosin

  • Renata Dabrowska
  • Ewa Próchniewicz
  • W. Drabikowski
Papers

DOI: 10.1007/BF00711959

Cite this article as:
Dabrowska, R., Próchniewicz, E. & Drabikowski, W. J Muscle Res Cell Motil (1983) 4: 83. doi:10.1007/BF00711959

Summary

F-actin filaments are disrupted by the action of cytochalasin and glutaraldehyde. Muscle tropomyosin which is able to polymerize can protect F-actin against fragmentation caused by these two agents. This protective effect does not occur with nonpolymerizable, brain or carboxypeptidase A-treated skeletal muscle tropomyosins. The protection of F-actin against the action of cytochalasin and glutaraldehyde takes place under conditions where the F-actin filaments are saturated with tropomyosin, that is, at a molar ratio of tropomyosin to actin of 1 : 7. It is suggested that nonpolymerizable tropomyosin lacks the protective ability because its binding to F-actin is considerably weaker than the polymerizable tropomyosin and does not saturate all of the binding sites on F-actin.

Copyright information

© Chapman and Hall Ltd. 1983

Authors and Affiliations

  • Renata Dabrowska
    • 1
  • Ewa Próchniewicz
    • 1
  • W. Drabikowski
    • 1
  1. 1.Department of Biochemistry of Nervous System and MuscleNencki Institute of Experimental BiologyWarsawPoland
  2. 2.Department of Biophysical Engineering, Faculty of Engineering SciencesOsaka UniversityToyonaka, OsakaJapan