The electric organ ofDiscopyge tschudii : Its innervated face and the biology of acetylcholinesterase
Rent the article at a discountRent now
* Final gross prices may vary according to local VAT.Get Access
An ultrastructural, histochemical, and biochemical study of the electric organ of the South American Torpedinid ray,Discopyge tschudii, was carried out.
Fine structural cytochemical localization of acetylcholinesterase (AChE) indicated that most of the esterase was associated with the basal lamina. Electron microscopy indicated no marked differences in the electrocyte ultrastructure betweenDiscopyge andTorpedo californica.
Discopyge electric organ possessed three molecular forms, two asymmetric forms (16 S and 13 S) and one globular hydrophobic form (6.5 S). The asymmetric 16 S AChE form was solubilized by heparin, a sulfated glycosaminoglycan, suggesting that heparin-like macromolecules are involved in the binding of the enzyme to the basal lamina.
Our results show that cell-free translated AChE peptides, synthesized usingDiscopyge electric organ poly (A+) RNA, correspond to a main band of 62,000 daltons which probably represents the catalytic subunit of the asymmetric AChE.
- The electric organ ofDiscopyge tschudii: Its innervated face and the biology of acetylcholinesterase
Cellular and Molecular Neurobiology
Volume 4, Issue 2 , pp 125-142
- Cover Date
- Print ISSN
- Online ISSN
- Kluwer Academic Publishers-Plenum Publishers
- Additional Links
- 16 S AChE
- synaptic basal lamina
- heparan sulfate proteoglycans
- cell-free synthesis of AChE
- electric organ
- Discopyge tschudii
- Industry Sectors
- Author Affiliations
- 1. Laboratory of Neurophysiology, Faculty of Biological Sciences, Catholic University of Chile, P.O. Box 114-D, Santiago, Chile
- 2. Laboratory of Histology, Faculty of Biological Sciences, Catholic University of Chile, P.O. Box 114-D, Santiago, Chile
- 3. Laboratory of Ecology, Faculty of Biological Sciences, Catholic University of Chile, P.O. Box 114-D, Santiago, Chile