Cellular and Molecular Neurobiology

, Volume 4, Issue 2, pp 125–142

The electric organ ofDiscopyge tschudii: Its innervated face and the biology of acetylcholinesterase

Authors

  • Bernardita Méndez
    • Laboratory of Neurophysiology, Faculty of Biological SciencesCatholic University of Chile
  • Jorge Garrido
    • Laboratory of Histology, Faculty of Biological SciencesCatholic University of Chile
  • Mafalda Maldonado
    • Laboratory of Neurophysiology, Faculty of Biological SciencesCatholic University of Chile
  • Fabián M. Jaksic
    • Laboratory of Ecology, Faculty of Biological SciencesCatholic University of Chile
  • Nibaldo C. Inestrosa
    • Laboratory of Neurophysiology, Faculty of Biological SciencesCatholic University of Chile
Article

DOI: 10.1007/BF00711000

Cite this article as:
Méndez, B., Garrido, J., Maldonado, M. et al. Cell Mol Neurobiol (1984) 4: 125. doi:10.1007/BF00711000

Summary

  1. 1.

    An ultrastructural, histochemical, and biochemical study of the electric organ of the South American Torpedinid ray,Discopyge tschudii, was carried out.

     
  2. 2.

    Fine structural cytochemical localization of acetylcholinesterase (AChE) indicated that most of the esterase was associated with the basal lamina. Electron microscopy indicated no marked differences in the electrocyte ultrastructure betweenDiscopyge andTorpedo californica.

     
  3. 3.

    Discopyge electric organ possessed three molecular forms, two asymmetric forms (16 S and 13 S) and one globular hydrophobic form (6.5 S). The asymmetric 16 S AChE form was solubilized by heparin, a sulfated glycosaminoglycan, suggesting that heparin-like macromolecules are involved in the binding of the enzyme to the basal lamina.

     
  4. 4.

    Our results show that cell-free translated AChE peptides, synthesized usingDiscopyge electric organ poly (A+) RNA, correspond to a main band of 62,000 daltons which probably represents the catalytic subunit of the asymmetric AChE.

     

Key words

16 S AChEsynaptic basal laminaheparan sulfate proteoglycanscell-free synthesis of AChEelectric organacetylcholinesteraseDiscopyge tschudii
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Copyright information

© Plenum Publishing Corporation 1984