Glycoconjugate Journal

, Volume 13, Issue 5, pp 823–831

Molecular characterization of the large heavily glycosylated domain glycopeptide from the rat small intestinal Muc2 mucin

Authors

  • Niclas G. Karlsson
    • Department of Medical BiochemistryGöteborg University
  • Malin Ev Johansson
    • Department of Medical BiochemistryGöteborg University
  • Noomi Asker
    • Department of Medical BiochemistryGöteborg University
  • Hasse Karlsson
    • Department of Medical BiochemistryGöteborg University
  • Sandra J. Gendler
    • Mayo Clinic Scottsdale
  • Ingemar Carlstedt
    • Department of Medical and Physiological Chemistry
  • Gunnar C. Hansson
    • Department of Medical BiochemistryGöteborg University
Papers

DOI: 10.1007/BF00702346

Cite this article as:
Karlsson, N.G., Johansson, M.E., Asker, N. et al. Glycoconjugate J (1996) 13: 823. doi:10.1007/BF00702346

Abstract

The largest high-glycosylated domain, glycopeptide A, of the ‘insoluble’ mucin complex of the rat small intestine has earlier been purified and characterized (Carlstedtet al., 1993,J Biol Chem268: 18771–81). A rabbit antiserum raised against deglycosylated glycopeptide A was used to clone part of a mucin showing homology to the human MUC2 mucin (Hanssonet al., 1994,Biochem Biophys Res Commun198: 181–90). This serum specifically stained goblet cells (paranuclear) in the mouse small intestine. The size of the coding sequence of glycopeptide A was estimated by using reversed transcriptase PCR of mRNA from an inbred rat strain (GOT-W) using primers in the unique central and C-terminal parts of the proposed rat Muc2 sequences. The PCR and Southern blot of the PCR products showed a fragment of about 5.5 kb corresponding to about 1700 amino acids when the known Cys-rich sequences used for the primers were subtracted. This is slightly larger than the size estimated earlier by biochemical studies. The mRNA encoding the rat Muc2 was slightly smaller than the mRNA encoding the human MUC2 in a colorectal cell line. Although the size of glycopeptide A estimated from biochemical results and by PCR is not identical, the results obtained here further support that the ‘insoluble’ mucin of the rat small intestine is encoded by the Muc2 gene. Most of the oligosaccharides in glycopeptide A were either neutral (40%) or sialylated (40%). The remaining ones were sulfated with the sulfate group attached to C-6 ofN-acetylglucosamine linked to C-6 of theN-acetylgalactosaminitol as revealed by tandem mass spectrometry of the perdeuteroacetylated oligosaccharides. Eighteen oligosaccharides were found of which fourteen were characterized and found to be mostly novel. Our findings thus expand the current knowledge of the core peptide of the rat intestinal goblet cell mucin and provide a relatively complete picture of the glycosylation of a defined mucin domain.

Keywords

mucinrat small intestinesulfated oligosaccharidesmmunolocalizationgoblet cellMuc2

Abbreviations

gpA

glycopeptide A

α-gpA

antiserum against glycopeptide A

CID

collision induced dissociation

FAB

fast atom bombardment

Hex

hexose

HexNAc

N-acetylhexosamine

HexNAcol

N-acetylhexosaminitol

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Copyright information

© Chapman & Hall 1996