Applied Physics B

, Volume 35, Issue 3, pp 135–140

A novel method of DNA transfection by laser microbeam cell surgery

  • M. Tsukakoshi
  • S. Kurata
  • Y. Nomiya
  • Y. Ikawa
  • T. Kasuya
Contributed Papers

DOI: 10.1007/BF00697702

Cite this article as:
Tsukakoshi, M., Kurata, S., Nomiya, Y. et al. Appl. Phys. B (1984) 35: 135. doi:10.1007/BF00697702

Abstract

A new technique is presented to incorporate exogeneous gene materials (DNA) into cells with a microbeam irradiation from an uv pulsed laser. A frequency-multiplied Nd:YAG laser, 355 m wavelength, 5 ns pulse duration, punches a self-healing hole of submicrometer aperture in cell membrane under selected irradiation conditions. It takes a fraction of a second for the aperture to close, long enough to allow the foreign DNA, contained in the medium, to slip into the cell. The method offers a clear advantage over existing methods: increases the success rate of DNA transfection as well as the efficiency of cell modification by orders of magnitude.

PACS

07.60 42.80 87 

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • M. Tsukakoshi
    • 1
  • S. Kurata
    • 1
  • Y. Nomiya
    • 1
  • Y. Ikawa
    • 1
  • T. Kasuya
    • 1
  1. 1.The Institute of Physical and Chemical ResearchWako, SaitamaJapan
  2. 2.Laboratory of Human Genetics, Medical Research InstituteTokyo Medical and Dental UniversityTokyoJapan