Acta Neuropathologica

, Volume 53, Issue 1, pp 21–28

Characterization of an established human malignant glioma cell line: LN-18

  • A. C. Diserens
  • N. de Tribolet
  • A. Martin-Achard
  • A. C. Gaide
  • J. F. Schnegg
  • S. Carrel
Original Works

DOI: 10.1007/BF00697180

Cite this article as:
Diserens, A.C., de Tribolet, N., Martin-Achard, A. et al. Acta Neuropathol (1981) 53: 21. doi:10.1007/BF00697180

Summary

A human malignant glioma cell line, LN-18, has been established in monolayer culture and subcultured for more than 115 passages. LN-18 cells grow in vitro as bipolar or stellate cells with pleomorphic nuclei, have a doubling time of about 72 h and a plating efficiency of 3%. The glial nature of these cells has been assessed by ultrastructural examination. The synthesis of glial fibrillary acidic and S-100 proteins could not be demonstrated, although the initial biopsy tissue and the early cultures were positive for the former. The presence of Ia-like antigens on the surface of these cells was demonstrated using allo and xeno antisera. LN-18 cells were also shown to synthesize large quantities of fibronectin. The injection of LN-18 cells into nude mice induced the formation of solid tumor masses that could be retransplanted every 3 weeks and showed a morphology comparable to that of the initial biopsy. Karyotype analysis revealed the presence of three marker chromosomes, constantly present before and after hetero-transplantation.

Key words

Malignant glioma Cell line LN-18 Human Surface antigens Chromosomes 

Abbreviations

GFA

glial acidic fibrillary protein

Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • A. C. Diserens
    • 1
  • N. de Tribolet
    • 1
  • A. Martin-Achard
    • 1
  • A. C. Gaide
    • 2
  • J. F. Schnegg
    • 1
  • S. Carrel
    • 3
  1. 1.Neurosurgical ServiceCHUVLausanneSwitzerland
  2. 2.Department of Medical GeneticsCHUVLausanneSwitzerland
  3. 3.Unit of Human Cancer Immunology, Lausanne BranchLudwig Institute for Cancer ResearchEpalingesSwitzerland

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