, Volume 99, Issue 1, pp 181-201

Ultrastructure ofCandida yeasts grown onn-alkanes

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Abstract

Catalase activities of the cells growing onn-alkanes of various strains ofCandida yeasts wer markedly higher than those of the cells growing on glucose, ethanol or acetate. In connection with this, electron-microscopical studies revealed abundant appearance of specific microbodies having homogeneous matrix surrounded by single unit membrane in the hydrocarbon-growing cells. Localization of catalase activity in the microbodies, in addition to the mitochondria, was confirmed by cytochemical treatment of the cells with 3,3′-diaminobenzidine reagent.

Inquiry on biochemical problems concerning this study should be addressed to Prof. Dr. Saburo Fukui, Department of Industrial Chemistry, Faculty of Engineering, Kyoto University, Sakyo-Ku, Kyoto, Japan. Inquiry on electronmicroscopical studies should be addressed to Dr. Masako Osumi, Department of Biology, Japan Women's University, Mejiro, Bunkyo-Ku, Tokyo, Japan.
Series of Yeast Microbodies. Part 1.