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The effect of sodium and magnesium and their interaction with quinine and lanthanum on spontaneous activity and related calcium movements of rat ileal smooth muscle

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Summary

  1. 1.

    Na-free media, with or without atropine, induced tonic contractures and enhanced spontaneous contractions of the rat ileum.

  2. 2.

    Quinine and lanthanum did not significantly inhibit these Na-free contractures, and neither agent, nor Na-free saline itself, caused any significant net gain of tissue calcium.

  3. 3.

    Na-free media enormously stimulated calcium efflux from the ileum whether atropine was present or not, suggesting a high tissue calcium turnover due to stimulation of surface transport sites. Quinine and lanthanum had no significant effect on this calcium efflux.

  4. 4.

    Mg-free media increased tension in the ileum and enhanced spontaneous contractions while high-Mg media inhibited contractility.

  5. 5.

    Mg-free media were without significant effect on calcium efflux from the ileum but they did induce a very small rise in tissue calcium.

  6. 6.

    These results are not consistent with the concept of a simple Na/Ca membrane counter exchange system in the ileum to regulate tissue calcium. On present evidence, a linked counter exchange system of Ca with Mg and Na would seem more likely.

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Authors and Affiliations

  1. Department of Biological Sciences, University of Lancaster, LA1 4YQ, Lancaster, England

    H. Huddart & K. H. M. Saad

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  1. H. Huddart
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  2. K. H. M. Saad
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Huddart, H., Saad, K.H.M. The effect of sodium and magnesium and their interaction with quinine and lanthanum on spontaneous activity and related calcium movements of rat ileal smooth muscle. J Comp Physiol B 126, 233–240 (1978). https://doi.org/10.1007/BF00688932

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  • DOI: https://doi.org/10.1007/BF00688932

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