Acta Neuropathologica

, Volume 71, Issue 3, pp 341–343

A specific histochemical marker (lectinRicinus communis agglutinin-1) for normal human microglia, and application to routine histopathology

  • H. Mannoji
  • H. Yeger
  • L. E. Becker
Short Original Communications

DOI: 10.1007/BF00688060

Cite this article as:
Mannoji, H., Yeger, H. & Becker, L.E. Acta Neuropathol (1986) 71: 341. doi:10.1007/BF00688060

Summary

Microglia were demonstrated in paraffinembedded human nervous tissues with an avidinbiotin peroxidase method andRicinus communis agglutinin-1 (RCA-1). Specific staining was observed in cell bodies and processes of microglia. Although endothelial cells and blood cells reacted with RCA-1, they were easily distinguished morphologically from microglia. Astrocytes, oligodendrocytes, and neurons did not react with RCA-1. These results suggest that RCA-1 can be used as a new histochemical marker for microglia in normal human brain.

Key words

Microglia Ricinus communis agglutinin-1 Lectin Histochemistry 

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • H. Mannoji
    • 1
  • H. Yeger
    • 1
  • L. E. Becker
    • 1
  1. 1.Division of NeuropathologyThe Hospital for Sick ChildrenTorontoCanada