Candidate metastasis-associated genes of the rat 13762NF mammary adenocarcinoma
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Differential hybridization was used to isolate genes potentially involved in the process of metastasis. Ten complementary DNAs (cDNAs) that were differentially expressed between a highly metastatic (MTLn3) and a nonmetastatic (MTC.4) line of the rat 13762NF mammary adenocarcinoma were isolated and sequenced. Examination of the EMBL/GenBank database revealed that one of the genes had a high degree of homology (98.8%) to annexin I (also known as calpactin II). Quantitative analysis of Northern blot hybridizations showed that the annexin I-like sequence was expressed 4- to 7-fold higher in MTLn3 than in MTC.4 cells. Steady state mRNA levels were also low in MTLn2, a cell line of low metastatic potential closely related to MTLn3, but were not related to metastatic potential in colon adenocarcinoma or melanoma cells. Two of the cDNAs (designated 8.11 and 10.14) were found to be novel. The expression of 10.14 mRNA (3.2 kb) was 4-fold higher in MTLn3 than in MTC.4 cells. Sequencing of the 10.14 cDNA (2.2 kb) revealed a putative open reading frame of 583 amino acids that was also novel. Expression of 8.11 mRNA (>7 kb) inversely correlated with metastatic potential. Another differentially transcribed gene was highly homologous to ERK2 (extracellular signal related kinase 2), a mitogen-activated protein kinase (MAPK). Northern analysis of ERK2 expression revealed 3-fold higher amounts of a 1.3 kb mRNA in MTLn3 than in MTC.4 cells. Higher levels of ERK2 mRNA were generally seen in the more metastatic human colon but not in melanoma cell lines. We also corroborated the work of Taniguchi (Nucl Acids Res 19:6949, 1991) by independently identifying EF-1α as a putative metastasis-associated gene.
- Dear TN, Kefford RF: Molecular oncogenetics of metastasis. Mol Aspects Med. 11:243–324, 1990.
- Sambrook J, Fritsch EF, Maniatis T: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.
- Ebralidze A, Tulchinsky E, Grigorian M, Afanasyeva A, Senin V, Revazova E, Lukanidin E: Isolation and characterization of a gene specifically expressed in different metastatic cells and whose deduced gene product has a high degree of homology to a Ca2+-binding protein family. Genes Devel 3:1086–1093, 1989.
- Steeg PS, Bevilacqua GB, Kopper L, Thorgeirsson UP, Talmadge JE, Liotta LA, Sobel ME: Evidence for a novel gene associated with low tumor metastatic potential. J Natl Cancer Inst 80:200–205, 1988.
- Dear TN, Ramshaw IA, Kefford RF: Differential expression of a novel gene, WDNM1, in nonmetastatic rat mammary adenocarcinoma cells. Cancer Res 48: 5203–5209, 1988.
- Dear TN, McDonald DA, Kefford RF: Transcriptional down-regulation of a rat gene, WDNM2, in metastatic DMBA-8 cells. Cancer Res 49:5323–5328, 1989.
- Phillips SM, Bendall AJ, Ramshaw IA: Isolation of a gene associated with high metastatic potential in rat mammary adenocarcinomas. J Natl Cancer Inst 82: 199–203, 1990.
- Basset P, Bellocq JP, Wolf C, Stoll I, Hutin P, Limacher JM, Podhajcer OL, Chenard MP, Rio MC, Chambon P: A novel metalloproteinase gene specifically expressed in stromal cells of breast carcinomas. Nature (London) 348:699–704, 1990.
- Welch DR, Neri A, Nicolson GL: Comparison of spontaneous and experimental metastasis using rat 13762 mammary adenocarcinoma metastatic cell clones. Invasion Metastasis 3:65–80, 1983.
- Neri A, Welch D, Kawaguchi T, Nicolson GL: Development and biologic properties of malignant cell sublines and clones of a spontaneously metastasizing rat mammary adenocarcinoma. J Natl Cancer Inst 68: 507–517, 1982.
- Nicolson GL: Differential organ tissue adhesion, invasion, and growth properties of metastatic rat mammary adenocarcinoma cells. Breast Cancer Res Treatment 12:167–176, 1988.
- Nicolson GL, Gallick GE, Dulski KM, Spohn WH, Lembo TM, Tainsky MA: Lack of correlation between intercellular junctional communication, p21rasEJ expression, and spontaneous metastatic properties of rat mammary cells after transfection with c-H-rasEJ or neo genes. Oncogene 5:747–753, 1990.
- Neri A, Nicolson GL: Phenotypic drift of metastatic and cell surface properties of mammary adenocarcinoma cell clones during growthin vitro. Int J Cancer 28:731–738, 1981.
- Welch DR, Drizman D, Nicolson GL: Multiple phenotypic divergence of mammary adenocarcinoma cell clones:in vitro andin vivo properties. Clin Exp Metastasis 2:333–355, 1984.
- Fidler IJ: Selection of successive tumor lines for metastasis. Nature (New Biol) 242:148–149, 1973.
- Morikawa K, Walker SM, Nakajima M: Influence of organ environment on the growth, selection, and metastasis of human colon carcinoma cells in nude mice. Cancer Res 48:6863–6871, 1988.
- Dworkin MB, Dawid IB: Use of a cloned library for the study of abundant poly-A+ RNA duringXenopus laevis development. Devel Biol 76:449–464, 1980.
- Yeatman TJ, Updyke TV, Kaetzel MA, Dedman JR, Nicolson GL: Expression of annexins on the surfaces of non-metastatic and metastatic human and rodent tumor cells. Clin Exp Metastasis 11:37–44, 1993
- Nakajima M, Welch DR, Belloni PN, Nicolson GL: Degradation of basement membrane type IV collagen and lung subendothelial matrix by rat mammary adenocarcinoma cell clones of differing metastatic potentials. Cancer Res 47: 4869–4867, 1987.
- Rozhin J, Wade RL, Honn KV, Sloane BF: Membrane-associated cathepsin L: A role in metastasis of melanomas. Biochem Biophys Res Commun 164:556–561, 1989.
- Yagel S, Warner AH, Nellans HN, Lala PK, Waghorne C, Denhardt DT: Suppression by cathepsin L inhibitors of the invasion of amnion membranes by murine cancer cells. Cancer Res 49:3553–3557, 1989.
- Lichtner RB, Moskwa PS, Nicolson GL: Heterogeneous expression of cytokeratins in metastatic mammary adenocarcinoma cellsin vitro andin vivo. Invasion Metastasis 7:367–383, 1987.
- Lichtner RB, Julian JA, North SM, Glasser SR, Nicolson GL: Coexpression of cytokeratins characteristic for adenocarcinoma tumors and their spontaneous metastases. Cancer Res 51:5943–5950, 1991.
- Chitpatima ST, Makrides S, Bandyopadhyay R, Brawerman G: Nucleotide sequence of a major messenger RNA for a 21 kilodalton polypeptide that is under translational control in mouse tumor cells. Nucl Acids Res 16:2350, 1988.
- Shimizu Y, Takabayashi E, Shinya Y, Shimizu N, Yamada, K, Gushima H: Molecular cloning and expression inEscherichia coli of the cDNA coding for rat lipocortin I (calpactin II). Gene 65:141–147, 1988.
- Kitajima Y, Owada MK, Mitsui H, Yaoita H: Lipocortin I (annexin I) is preferentially localized on the plasma membrane in keratinocytes of psoriatic lesional epidermis as shown by immunofluorescence microscopy. J Invest Dermatol 97:1032–1038, 1991.
- Campos-Gonzalez R, Kanemitsu M, Boynton AL: Epidermal growth factor induces the accumulation of calpactin II on the cell surface during membrane ruffling. Cell Motil Cytoskel 15:34–40, 1990.
- Barnes JA, Michiel D, Hollenberg MD: Simultaneous phosphorylation of three human calpactins by protein kinase C. Biochem Cell Biol 69:163–169, 1991.
- Horlick KR, Ganjianpour M, Frost SC, Nick HS: Annexin-I regulation in response to suckling and rat mammary cell differentiation. Endocrinology 128: 1574–1579, 1991.
- Edashige K, Utsumi T, Sato EF, Ide A, Kasai M: Requirement of protein association with membranes for phosphorylation by protein kinase C. Arch Biochem Biophys 296:296–301, 1992.
- Creutz CE: The annexins and exocytosis. Science 258:294–931, 1992.
- Tressler RJ, Updyke TV, Yeatman T, Nicolson GL: Extracellular Annexin II is associated with divalent cation-dependent tumor cell-endothelial cell adhesion. Proc Natl Acad Sci USA (in press).
- Brands JH, Maassen JA, Van Hemert FJ, Amons R, Moller W: The primary structure of the alpha subunit of human elongation factor 1. Eur J Biochem 155: 167–171, 1986.
- Yang F, Demma M, Warren V, Dharmawardhane S, Condeelis, J: Identification of an actin-binding protein fromDictyostelium as elongation factor 1α. Nature 347:494–496, 1990.
- Taniguchi S, Miyamoto S, Sadano H, Kobayashi H: Rat elongation factor 1 alpha: sequence of cDNA from a highly metastatic fos-transferred cell line. Nucleic Acids Res 19:6949, 1992.
- Jones JG, Segall J, Condeelis J: Molecular analysis of amoeboid chemotaxis: parallel observations in amoeboid phagocytes and metastatic tumor cells. Experientia (Suppl) 59:1–16, 1991.
- Gotoh Y, Nishida E, Yamashita T, Hoshi M, Kawakami M: Microtubule-associated-protein MAP kinase activated by nerve growth factor and epidermal growth factor in PC12 cells. Identity with the mitogenactivated MAP kinase of fibroblastic cells. Eur J Biochem 193: 661–669, 1990.
- Crew CM, Alessandrini A, Erikson RL: Erks: Their fifteen minutes has arrived. Cell Growth Diff 3:135–142, 1992.
- Boulton TG, Nye SH, Robbins DJ, Ip NY, Radziejewska E, Morgenbesser SD, DePinho RA, Panaytatos N, Cobb MH, Yancopoulos GD: ERKs: A family of protein-serine/threonine kinases that are activated and tyrosine phosphorylated in response to insulin and NGF. Cell 65:663–675, 1991.
- Thomas SM, DeMarco M, D'Arcangelo G, Halegoua S, Brugge JS: Ras is essential for nerve growth factor and phorbol ester-induced tyrosine phosphorylation of MAP kinases. Cell 68:1031–1040, 1992.
- L'Allemain G, Pouyssegur J, Weber MJ: p42/mitogen-activated protein kinase as a converging target for different growth factor signaling pathways: use of pertussis toxin as a discrimination factor. Cell Regulation 2:675–684, 1991.
- Stackpoole CW: Distinct lung-colonizing and lung metastasizing cell populations in B 16 mouse melanoma. Nature (London) 289:798–800, 1981.
- Tobe K, Kadowaki T, Tamemoto H, Ueki K, Hara K, Koshio O, Momomura K, Gotoh Y, Nishida E, Akanuma Y: Insulin and 12-O-tetradecanoylphorbol-13-acetate activation of two immunologically distinct myelin basic protein/microtubule-associated protein 2 MBP/MAP2 kinases via de novo phosphorylation of threonine and tyrosine residues. J Biol Chem 266: 24793–24803, 1991.
- Korczak B, Whale C, Kerbel RS: Possible involvement of Ca2+ mobilization and protein kinase C activation in the induction of spontaneous metastasis by mouse mammary adenocarcinoma cells. Cancer Res 49:2597–2602, 1989.
- Gopalakrishna R, Barsky SH: Tumor promoter-induced membrane bound protein kinase C regulates hematogenous metastasis. Proc Natl Acad Sci USA 85:612–616, 1988.
- Schwartz GK, Redwood SM, Ohnuma T, Holland JF, Droller MJ, Liu BC-S: Inhibition of invasion of invasive human bladder carcinoma cells by protein kinase C inhibitor staurosporine. J Natl Cancer Inst 82:1753–1756, 1990.
- Huhtala P, Chow LT, Tryggvason K: Structure of the human type IV collagenase gene. J Biol Chem 265: 11077–11082, 1990.
- Chauhan SS, Goldstein LJ, Gottesman MM: Expression of cathepsin L in human tumors. Cancer Res 51: 1478–1481, 1991.
- Candidate metastasis-associated genes of the rat 13762NF mammary adenocarcinoma
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