Molecular and General Genetics MGG

, Volume 222, Issue 2, pp 323–328

Alcohol dehydrogenase III inAspergillus nidulans is anaerobically induced and post-transcriptionally regulated

  • Joan M. Kelly
  • Mary R. Drysdale
  • Heather M. Sealy-Lewis
  • I. Gwyn Jones
  • Robin A. Lockington
Article

DOI: 10.1007/BF00633836

Cite this article as:
Kelly, J.M., Drysdale, M.R., Sealy-Lewis, H.M. et al. Mol Gen Genet (1990) 222: 323. doi:10.1007/BF00633836

Summary

An alcohol dehydrogenase was shown to be induced inAspergillus nidulans by periods of anaerobic stress. This alcohol dehydrogenase was shown to correspond to the previously described cryptic enzyme, alcohol dehydrogenase III (McKnight et al. 1985), by analysis of a mutation in the structural gene of alcohol dehydrogenase III,alcC, created by gene disruption. Survival tests on agar plates showed that this enzyme is required for long-term survival under anaerobic conditions. Northern blot analysis and gene fusion studies showed that the expression of thealcC gene is regulated at both the transcriptional and translational levels. Thus there are mechanisms in this filamentous fungus allowing survival under anaerobic stress that are similar to those described in higher plants.

Key words

Alcohol dehydrogenase III Anaerobic stress Aspergillus nidulans Filamentous fungi Post-transcriptional regulation 

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Joan M. Kelly
    • 1
  • Mary R. Drysdale
    • 1
  • Heather M. Sealy-Lewis
    • 2
  • I. Gwyn Jones
    • 2
  • Robin A. Lockington
    • 3
  1. 1.Department of GeneticsUniversity of AdelaideAdelaideAustralia
  2. 2.Department of Applied BiologyUniversity of HullHullUK
  3. 3.Department of Animal ScienceUniversity of AdelaideAdelaideAustralia

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