Molecular and General Genetics MGG

, Volume 222, Issue 2, pp 265–269

Nucleotide sequence of thecelA gene encoding a cellodextrinase ofRuminococcus flavefaciens FD-1

  • Wenyen Wang
  • Jennifer A. Thomson
Article

DOI: 10.1007/BF00633827

Cite this article as:
Wang, W. & Thomson, J.A. Mol Gen Genet (1990) 222: 265. doi:10.1007/BF00633827

Summary

The nucleotide sequence of a 3.6 kb DNA fragment containing a cellodextrinase gene (celA) fromRuminococcus flavefaciens FD-1 was determined. The gene was expressed from its own regulatory region inEscherichia coli and a putative consensus promoter sequence was identified upstream of a ribosome binding site and a TTG start codon. The complete amino acid sequence of the CeIA enzyme (352 residues) was deduced and showed no significant homology to cellulases from other oganisms. Two lysozymetype active sites were found in the amino-terminal third of the enzyme. InE. coli the cloned CeIA protein was translocated into the periplasm. The lack of a typical signal sequence, and the results of transposonphoA mutagenesis experiments indicated that CeIA is secreted by a mechanism other than a leader peptide.

Key words

Ruminococcus flavefaciens Cellodextrinase Sequence Secretion 

Abbreviations

CMCase

carboxymethylcellulase

celA

gene coding for CeIA

CelA

cellodextrinase

ORF

open reading frame

phoA

gene encoding alkaline phosphatase

pNPC

p-nitrophenyl-β-d-cellobioside

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Wenyen Wang
    • 1
  • Jennifer A. Thomson
    • 1
  1. 1.Department of MicrobiologyUniversity of Cape TownRondeboschSouth Africa