Planta

, Volume 198, Issue 3, pp 371–377

Chilling, oxidative stress and antioxidant responses inArabidopsis thaliana callus

  • Damian O'Kane
  • Vera Gill
  • Patricia Boyd
  • Roy Burdon
Article

DOI: 10.1007/BF00620053

Cite this article as:
O'Kane, D., Gill, V., Boyd, P. et al. Planta (1996) 198: 371. doi:10.1007/BF00620053

Abstract

Chilling ofArabidopsis thaliana (L.) Heynh. callus tissue to 4 °C led to conditions of oxidative stress, as indicated by increased levels of the products of peroxidative damage to cell membranes. Cellular H2O2 was also observed to increase initially upon chilling but by day 8 cellular levels had declined to below control levels. Although levels of catalase activity remained similar to those in unchilled tissue, activity of ascorbate peroxidase increased between days 4 and 8 of chilling to 4 °C. In callus held at 23 °C, levels of reduced glutathione remained static whereas they rose in callus held at 4 °C. Levels of oxidised glutathione were initially low but increased significantly by day 4 in the chilled callus. At 23 °C, however, levels of oxidised glutathione remained low. Between days 1 and 3 at 4 °C, levels of glutathione reductase activity increased but by day 8 glutathione reductase activity was similar to that in cells held at 23 °C. Exposure of callus to abscisic acid at 23 °C also led to increased activities of ascorbate peroxidase and glutathione reductase.

Key words

Antioxidant enzymes Arabidopsis Chilling stress Glutathione Hydrogen peroxide Lipid peroxidation 

Abbreviations

ABA

abscisic acid

GSH

reduced glutathione

GSSG

oxidised glutathione

TTC

2′,3′5′-triphenyltetrazolium chloride

Copyright information

© Springer-Verlag 1996

Authors and Affiliations

  • Damian O'Kane
    • 1
  • Vera Gill
    • 1
  • Patricia Boyd
    • 1
  • Roy Burdon
    • 1
  1. 1.Department of Bioscience and BiotechnologyUniversity of StrathclydeGlasgowUK