Chemistry of Natural Compounds

, Volume 25, Issue 1, pp 45-49

Study of carbohydrase-catalyzed reactions with the aid of EDIAP mass spectrometry

  • P. V. Bezukladnikov
  • , L. A. Elyakova
  • , T. N. Zvyagintseva
  • , O. A. Mirgorodskaya

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The distribution of the products of the enzymatic transformation of various substrates under the action of the endo-β-(1 → 3)-glucanase L IV from the molluscSpisula sachalinensis has been investigated and the possibility has been shown of the simultaneous recording, with the aid of EDIAP mass spectrometry, of the products of hydrolysis and of transglycosylation. It has been shown that methyl gentiobioside and methyl cellobioside are acceptors in the transglycosylation reaction, as a result of which glucooligosaccharides with a mixed type of glycosidic bonds are formed. The ratio of mono-, di-, tri-, and tetrasaccharides labeled at the ends with ethylene glycol formed in the enzymatic hydroysis of laminarin previously subjected to Smith degradation has been determined.