Pflügers Archiv

, Volume 427, Issue 1, pp 102–109

The distribution of intracellular calcium concentration in isolated single fibres of mouse skeletal muscle during fatiguing stimulation

  • S. Duty
  • D. G. Allen
Heart, Circulation, Respiration and Blood; Environmental and Exercise Physiology

DOI: 10.1007/BF00585948

Cite this article as:
Duty, S. & Allen, D.G. Pflügers Arch. (1994) 427: 102. doi:10.1007/BF00585948

Abstract

Fluorescence microscopy has been used to examine the distribution of intracellular calcium concentration ([Ca2+]i) in isolated single fibres from the mouse flexor brevis muscle during fatiguing stimulation. Under control conditions there was a virtually uniform distribution of [Ca2+]i in fura-2 loaded fibres either at rest or during short (0.35 s, 100 Hz) tetani. Fatigue produced by repeated short tetani was accompanied by an early rise, followed by a marked fall, in tetanic [Ca2+]i. Throughout the period of fatiguing stimulation the distribution of [Ca2+]i remained uniform with no detectable gradients observed. In contrast, when fatigue was produced by continuous 100 Hz stimulation, a small gradient of [Ca2+]i developed across the fibre with the [Ca2+]i in the centre of the fibre lower than that at the edge of the fibre. This gradient was apparent after 1.7 s, persisted for at least 11 s and was superimposed on a rise followed by a fall in spatially averaged [Ca2+]i. Reduction of the extracellular Na+ to 50% caused reduced force production and a reduced [Ca2+]i in the centre of the fibre. To assess the contribution of reduced response of the myofibrillar proteins to [Ca2+]i during continuous tetani, the relation between [Ca2+]i and force throughout the long tetanus was compared with that obtained in short, unfatigued tetani. These results show that in long tetani, reduced tetanic [Ca2+]i and reduced responsiveness of the myofibrillar proteins to [Ca2+]i each make important contributions to the decline of force, whereas the gradients of [Ca2+]i make only a small contribution.

Key words

Skeletal muscle Fatigue Intracellular Calcium Fluorescent imaging 

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • S. Duty
    • 1
  • D. G. Allen
    • 1
  1. 1.Department of PhysiologyUniversity of SydneyAustralia
  2. 2.Department of Cell and Structural BiologyUniversity of ManchesterManchesterUK